Isolation and genome characterization of Lloviu virus from Italian Schreibers's bats.
Isolation and genome characterization of Lloviu virus from Italian Schreibers's bats.
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DOI：
10.1038/s41598-023-38364-7
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发表时间：
2023-07-13
影响因子：
4.6
通讯作者：
Kemenesi, Gabor
中科院分区：
文献类型：
--
作者：
Toth, Gabor E.;Hume, Adam J.;Suder, Ellen L.;Zeghbib, Safia;abraham, Agota;Lanszki, Zsofia;Varga, Zsaklin;Tauber, Zsofia;Foeldes, Fanni;Zana, Brigitta;Scaravelli, Dino;Scicluna, Maria Teresa;Pereswiet-Soltan, Andrea;Goerfoel, Tamas;Terregino, Calogero;De Benedictis, Paola;Garcia-Dorival, Isabel;Alonso, Covadonga;Jakab, Ferenc;Muehlberger, Elke;Leopardi, Stefania;Kemenesi, Gabor
Lloviu cuevvirus （LLOV）是在埃博拉病毒和马尔堡病毒属之外发现的首个丝状病毒科成员。2002年，在伊比利亚半岛，一种名为Schreibers的蝙蝠（Miniopterus schreibersii）大规模死亡，导致了它的首次发现。最近对重组和野生型LLOV分离株的研究证实了该病毒在体外的人畜共患性。我们检查了来自意大利的蝙蝠样本，在该病毒目前已知分布范围之外的地区是否存在LLOV。我们检测到一份2020年的阳性样本，对病毒基因组的完整编码区进行了测序，并建立了该病毒的传染性分离物。此外，我们利用西班牙、匈牙利和意大利的序列，对病毒进行了首次全面的进化分析。本研究最重要的成果是使用SuBK12-08细胞从蝙蝠样本中建立了另一种传染性LLOV分离物，表明该细胞系对LLOV感染高度敏感，并证实了先前的观察，即这些蝙蝠在本质上是病毒的有效宿主。这一结果进一步加强了蝙蝠作为人畜共患丝状病毒天然宿主的作用。
Lloviu cuevavirus (LLOV) was the first identified member of Filoviridae family outside the Ebola and Marburgvirus genera. A massive die-off of Schreibers’s bats (Miniopterus schreibersii) in the Iberian Peninsula in 2002 led to its initial discovery. Recent studies with recombinant and wild-type LLOV isolates confirmed the zoonotic nature of the virus in vitro. We examined bat samples from Italy for the presence of LLOV in an area outside of the currently known distribution range of the virus. We detected one positive sample from 2020, sequenced the complete coding region of the viral genome and established an infectious isolate of the virus. In addition, we performed the first comprehensive evolutionary analysis of the virus, using the Spanish, Hungarian and the Italian sequences. The most important achievement of this study is the establishment of an additional infectious LLOV isolate from a bat sample using the SuBK12-08 cells, demonstrating that this cell line is highly susceptible to LLOV infection and confirming the previous observation that these bats are effective hosts of the virus in nature. This result further strengthens the role of bats as the natural hosts for zoonotic filoviruses.
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影响因子：
4.7
作者：
Ramirez de Arellano, Eva;Sanchez-Lockhart, Mariano;Negredo, Anabel
通讯作者：
Negredo, Anabel
影响因子：
28.3
作者：
Goldstein T;Anthony SJ;Gbakima A;Bird BH;Bangura J;Tremeau-Bravard A;Belaganahalli MN;Wells HL;Dhanota JK;Liang E;Grodus M;Jangra RK;DeJesus VA;Lasso G;Smith BR;Jambai A;Kamara BO;Kamara S;Bangura W;Monagin C;Shapira S;Johnson CK;Saylors K;Rubin EM;Chandran K;Lipkin WI;Mazet JAK
通讯作者：
Mazet JAK
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
DOI：
10.3390/v13102064
发表时间：
2021-10-14
期刊：
Viruses
影响因子：
--
作者：
Leopardi S;Barneschi E;Manna G;Zecchin B;Priori P;Drzewnioková P;Festa F;Lombardo A;Parca F;Scaravelli D;Maroni Ponti A;De Benedictis P
通讯作者：
De Benedictis P
影响因子：
5
作者：
Bellizzi, Saverio;Pichierri, Giuseppe;Popescu, Christian
通讯作者：
Popescu, Christian