Tissue culture protocols for the obligate parasitic plant Striga hermonthica and implications for host-parasite co-cultivation
Tissue culture protocols for the obligate parasitic plant Striga hermonthica and implications for host-parasite co-cultivation
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专性寄生植物独脚金的组织培养方案及其对宿主-寄生虫共培养的影响
DOI：
10.1007/s11240-019-01621-7
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Waweru D
中科院分区：
文献类型：
--
作者：
Waweru D
独脚金是一种寄生植物，它限制了主粮谷物的生产，对撒哈拉以南非洲数百万小农的生计构成了巨大威胁。寄生虫附着在作物的根部，并与宿主的木质部血管建立血管连接，以便获取水、有机和无机养分，导致受感染植物生长发育不良和死亡。宿主与寄生虫相互作用的机制尚不清楚，这使得控制寄生虫的明智策略变得困难。为了促进分子和遗传学研究中独脚金与宿主相互作用的研究，我们：（i）建立了从组织培养种子中生长独脚金的有效方案； (ii) 通过直接器官发生和体细胞胚胎发生优化其再生方案，以及 (iii) 确定在相同培养基中共培养宿主和寄生虫的效果。我们发现最佳的生长素和细胞分裂素浓度是：用于胚胎愈伤组织再生的 10.7 μM 萘乙酸 (NAA) 和 2.2 μM 6-苄氨基嘌呤 (BAP)，以及用于芽增殖的 1.1–4.4 μM BAP（不含 NAA）。虽然幼苗、茎和叶外植体以相同的频率诱导愈伤组织，但种子胚根不产生任何愈伤组织。出乎意料的是，我们发现，当在培养基上生长的水稻中添加独脚金时，寄生虫通过在叶子上形成病变对宿主产生不利影响，并导致寄生虫中愈伤组织的芽诱导减少。本研究中描述的技术将增强对独脚金与宿主相互作用的进一步了解。
Strigais a genus of parasitic plants that poses great danger to livelihoods of millions of smallholder farmers in sub-Saharan Africa by limiting production of staple cereals. The parasite attaches to the roots of the crop and establishes a vascular connection with the host’s xylem vessels in order to access water, organic and inorganic nutrients, leading to stunted growth and death of the infected plant. Mechanisms underpinning host–parasite interactions are not clearly understood making well-informed strategies for control of the parasite difficult. To facilitate studies ofStriga–host interactions for molecular and genetic studies, we: (i) established an efficient protocol for growingStrigafrom seeds in tissue culture; (ii) optimized protocols for its regeneration through direct organogenesis and somatic embryogenesis, and (iii) determined the effect of co-culturing host and parasite in the same culture media. We found the best auxin and cytokinin concentrations to be: 10.7 μM naphthaleneacetic acid (NAA) and 2.2 μM 6-benzylaminopurine (BAP) for embryogenic callus regeneration and 1.1–4.4 μM BAP without NAA for shoot multiplication. While seedling, stem and leaf explants induced callus with the same frequency, seed radicles did not produce any callus. Unexpectedly, we found that whenStrigacallus was added in rice growing on culture media, the parasite adversely affected the host through formation of lesions on leaves and resulted in less shoot induction from callus in the parasite. Techniques described in this study will enhance further understanding ofStriga–host interactions.
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影响因子：
2.3
作者：
Ejeta, Gebisa
通讯作者：
Ejeta, Gebisa
DOI：
10.1007/s11240-018-1466-x
发表时间：
2018-08
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
Dagmara Libiaková;C. Ruyter-Spira;H. Bouwmeester;R. Matusova
通讯作者：
Dagmara Libiaková;C. Ruyter-Spira;H. Bouwmeester;R. Matusova
影响因子：
6.9
作者：
CAI, T;BABIKER, AG;BUTLER, LG
通讯作者：
BUTLER, LG
影响因子：
5.1
作者：
Fernández-Aparicio M;Rubiales D;Bandaranayake PC;Yoder JI;Westwood JH
通讯作者：
Westwood JH
影响因子：
6.6
作者：
De Groote, Hugo;Wangare, Lucy;Friesen, Dennis
通讯作者：
Friesen, Dennis