Schistosoma japonicum Infection Leads to the Reprogramming of Glucose and Lipid Metabolism in the Colon of Mice.
Schistosoma japonicum Infection Leads to the Reprogramming of Glucose and Lipid Metabolism in the Colon of Mice.
复制标题
日本血吸虫感染导致小鼠结肠中葡萄糖和脂质代谢的重新编程
DOI：
10.3389/fvets.2021.645807
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发表时间：
2021
影响因子：
3.2
通讯作者：
Pan W
中科院分区：
文献类型：
--
作者：
Yang X;Ding W;Qian X;Jiang P;Chen Q;Zhang X;Lu Y;Wu J;Sun F;Pan Z;Li X;Pan W
日本血吸虫（S. japonicum）虫卵在结肠内的沉积常引起炎症、纤维化、增生、溃疡和息肉病，对人体健康构成严重威胁。然而，潜在的机制在很大程度上被忽视了。近年来，糖脂代谢紊乱参与了寄生虫诱导的肝纤维化，为研究该病肠道病理的潜在机制提供了新的线索。本研究的重点是日本血吸虫感染小鼠结肠中葡萄糖和脂质代谢重编程谱。我们发现日本血吸虫感染使结肠长度缩短，肠道完整性受损，诱导蛋状肉芽肿形成，并增加结肠炎症。在感染小鼠的结肠中，参与调节糖脂代谢途径的关键酶的表达上调。相反，10号染色体上缺失的磷酸酶和紧张素同源物（PTEN）及其下游信号靶点在感染后明显受到抑制。与这些结果一致，可溶性卵抗原（SEA）的体外刺激下调了CT-26细胞中PTEN的表达，并诱导了与体内结果相似的代谢改变。此外，PTEN过表达可阻止SEA诱导的CT-26细胞糖脂代谢重编程。综上所述，本研究表明日本血吸虫感染诱导小鼠结肠糖脂代谢重编程，PTEN可能在介导这种代谢重编程中发挥重要作用。这些发现为日本血吸虫在宿主中的致病性提供了新的见解。
The deposition of Schistosoma japonicum (S. japonicum) eggs commonly induces inflammation, fibrosis, hyperplasia, ulceration, and polyposis in the colon, which poses a serious threat to human health. However, the underlying mechanism is largely neglected. Recently, the disorder of glucose and lipid metabolism was reported to participate in the liver fibrosis induced by the parasite, which provides a novel clue for studying the underlying mechanism of the intestinal pathology of the disease. This study focused on the metabolic reprogramming profiles of glucose and lipid in the colon of mice infected by S. japonicum. We found that S. japonicum infection shortened the colonic length, impaired intestinal integrity, induced egg-granuloma formation, and increased colonic inflammation. The expression of key enzymes involved in the pathways regulating glucose and lipid metabolism was upregulated in the colon of infected mice. Conversely, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and its downstream signaling targets were significantly inhibited after infection. In line with these results, in vitro stimulation with soluble egg antigens (SEA) downregulated the expression of PTEN in CT-26 cells and induced metabolic alterations similar to that observed under in vivo results. Moreover, PTEN over-expression prevented the reprogramming of glucose and lipid metabolism induced by SEA in CT-26 cells. Overall, the present study showed that S. japonicum infection induces the reprogramming of glucose and lipid metabolism in the colon of mice, and PTEN may play a vital role in mediating this metabolic reprogramming. These findings provide a novel insight into the pathogenicity of S. japonicum in hosts.
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影响因子：
7.3
作者：
Hams E;Aviello G;Fallon PG
通讯作者：
Fallon PG
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
7.3
作者：
Schwartz C;Fallon PG
通讯作者：
Fallon PG
日本血吸虫感染后吡喹酮治疗可维持小鼠肝脏胰岛素敏感性并改善葡萄糖代谢。
影响因子：
3.2
作者：
Luo X;Zhu Y;Liu R;Song J;Zhang F;Zhang W;Xu Z;Hou M;Yang B;Chen L;Ji M
通讯作者：
Ji M
影响因子：
16.6
作者：
Mayer JU;Demiri M;Agace WW;MacDonald AS;Svensson-Frej M;Milling SW
通讯作者：
Milling SW