Morphological and Biomechanical Differences in the Elastase and AngII apoE(-/-) Rodent Models of Abdominal Aortic Aneurysms.
Morphological and Biomechanical Differences in the Elastase and AngII apoE(-/-) Rodent Models of Abdominal Aortic Aneurysms.
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DOI：
10.1155/2015/413189
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发表时间：
2015
影响因子：
--
通讯作者：
Goergen CJ
中科院分区：
文献类型：
--
作者：
Phillips EH;Yrineo AA;Schroeder HD;Wilson KE;Cheng JX;Goergen CJ
腹主动脉瘤（AAA）是一种具有多因素发展和进展的潜在致命性心血管疾病。已经开发了两种疾病的临床前模型（载脂蛋白E缺陷动物中的弹性蛋白酶灌注和血管紧张素II输注）来研究疾病的开始和进展。到目前为止，大多数研究都使用离体方法来检查疾病特征，如扩张的主动脉直径或分析方法来观察循环生物标志物。在此，我们提供的证据，从体内超声研究中发生的时间变化的生物力学参数和大分子的主动脉壁在每个模型。我们目前的研究结果来自28天的弹性蛋白酶灌注大鼠和AngII apoE−/−小鼠。虽然每个模型都开发了特定于其诱导方法的AAA，但它们都具有与人类动脉瘤相同的特征，例如血管应变和血流速度的显著变化。组织学和非线性显微镜证实，弹性蛋白和胶原蛋白，都是重要的细胞外基质分子，在其水平和空间分布受到类似的影响。未来的研究可以利用这些模型之间的差异，以研究疾病进展的机制或评估潜在的AAA治疗。
An abdominal aortic aneurysm (AAA) is a potentially fatal cardiovascular disease with multifactorial development and progression. Two preclinical models of the disease (elastase perfusion and angiotensin II infusion in apolipoprotein-E-deficient animals) have been developed to study the disease during its initiation and progression. To date, most studies have used ex vivo methods to examine disease characteristics such as expanded aortic diameter or analytic methods to look at circulating biomarkers. Herein, we provide evidence from in vivo ultrasound studies of the temporal changes occurring in biomechanical parameters and macromolecules of the aortic wall in each model. We present findings from 28-day studies in elastase-perfused rats and AngII apoE−/− mice. While each model develops AAAs specific to their induction method, they both share characteristics with human aneurysms, such as marked changes in vessel strain and blood flow velocity. Histology and nonlinear microscopy confirmed that both elastin and collagen, both important extracellular matrix molecules, are similarly affected in their levels and spatial distribution. Future studies could make use of the differences between these models in order to investigate mechanisms of disease progression or evaluate potential AAA treatments.
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影响因子：
4.4
作者：
Goergen, Craig J.;Barr, Kyla N.;Huynh, Diem T.;Eastham-Anderson, Jeffrey R.;Choi, Gilwoo;Hedehus, Maj;Dalman, Ronald L.;Connolly, Andrew J.;Taylor, Charles A.;Tsao, Philip S.;Greve, Joan M.
通讯作者：
Greve, Joan M.
影响因子：
5.6
作者：
Cao RY;Amand T;Ford MD;Piomelli U;Funk CD
通讯作者：
Funk CD
影响因子：
15.9
作者：
Longo, GM;Xiong, WF;Baxter, BT
通讯作者：
Baxter, BT
影响因子：
7.5
作者：
Buxton, Denis B.
通讯作者：
Buxton, Denis B.
影响因子：
39.2
作者：
Lederle, FA;Johnson, GR;Ballard, DJ
通讯作者：
Ballard, DJ