Autoclaving of Poloxamer 407 hydrogel and its use as a drug delivery vehicle.
Autoclaving of Poloxamer 407 hydrogel and its use as a drug delivery vehicle.
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泊洛沙姆407水凝胶的高压灭菌及其作为药物递送载体的用途。
DOI：
10.1002/jbm.b.34703
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Priddy LB
中科院分区：
文献类型：
--
作者：
Beard MC;Cobb LH;Grant CS;Varadarajan A;Henry T;Swanson EA;Kundu S;Priddy LB
随着耐药细菌的日益普遍，迫切需要能够靶向、原位给药的生物材料。合成聚合物poloxam407 （P407）由于其热可逆凝胶化而引起特别的兴趣。P407的临床应用通常涉及高压灭菌，但这些极端环境条件对水凝胶含水量、流变性能和作为药物递送载体的功效的影响尚不清楚。本研究的目的是研究高压灭菌对P407水凝胶性能的影响。高压灭菌由于蒸发减少了水凝胶的含水量，从而增加了水凝胶的聚合物重量分数。相比之下，除了在高压灭菌后凝胶化温度降低外，高压灭菌的水凝胶与非高压灭菌的水凝胶具有相似的流变特性。体外高压灭菌不影响水凝胶作为万古霉素抗生素载体的作用，P407（含和不含万古霉素）对浮游金黄色葡萄球菌有杀菌作用。一项使用P407递送噬菌体的体内试点研究强调了对外科应用水凝胶功能的进一步了解的必要性。综上所述，高压灭菌降低了P407水凝胶的含水量和凝胶温度，而其他流变性能和作为万古霉素体外递送载体的生物材料的功效不受影响。
With antibiotic-resistant bacteria becoming increasingly prevalent, biomaterials capable of targeted, in situ drug delivery are urgently needed. The synthetic polymer Poloxamer 407 (P407) is of particular interest due to its thermoreversible gelation. Clinical use of P407 typically involves sterilization via autoclaving, but the effects of these extreme environmental conditions on hydrogel water content, rheological properties and efficacy as a drug delivery vehicle remain unknown. The aim of this study was to investigate the effects of autoclaving on the properties of P407 hydrogel. Autoclaving reduced hydrogel water content due to evaporation, thus increasing the polymer weight fraction of the hydrogels. In contrast, except for a reduction in gelation temperature following autoclaving, autoclaved hydrogels had similar rheological properties as nonautoclaved hydrogels. In vitro, autoclaving did not hinder the hydrogel’s efficacy as a carrier for vancomycin antibiotic, and P407 (with and without vancomycin) had a bactericidal effect on planktonic Staphylococcus aureus. An in vivo pilot study using P407 to deliver bacteriophage highlighted the need for additional understanding of the functionality of the hydrogel for surgical applications. In conclusion, P407 hydrogel water content and gelation temperature were reduced by autoclave sterilization, while other rheological properties and the efficacy of the biomaterial as a delivery vehicle for vancomycin in vitro were unaffected.
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影响因子：
4.9
作者：
Veyries, ML;Faurisson, F;Rouveix, B
通讯作者：
Rouveix, B
DOI：
10.1016/j.jconrel.2015.12.044
发表时间：
2016-02-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Manda P;Kushwaha AS;Kundu S;Shivakumar HN;Jo SB;Murthy SN
通讯作者：
Murthy SN
影响因子：
4.6
作者：
Jabarian, Laleh Erfani;Rouini, Mohammad Reza;Dinarvand, Rassoul
通讯作者：
Dinarvand, Rassoul
影响因子：
3.8
作者：
Karajanagi SS;Yoganathan R;Mammucari R;Park H;Cox J;Zeitels SM;Langer R;Foster NR
通讯作者：
Foster NR
DOI：
10.1016/j.jtcvs.2006.04.028
发表时间：
2006-11-01
影响因子：
6
作者：
Bouchot, Olivier;Aubin, Marie-Claude;Perrault, Louis P.
通讯作者：
Perrault, Louis P.