Functional Analysis of the Chaperone-Usher Fimbrial Gene Clusters of Salmonella enterica serovar Typhi.
Functional Analysis of the Chaperone-Usher Fimbrial Gene Clusters of Salmonella enterica serovar Typhi.
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DOI：
10.3389/fcimb.2018.00026
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发表时间：
2018
影响因子：
5.7
通讯作者：
Daigle F
中科院分区：
文献类型：
--
作者：
Dufresne K;Saulnier-Bellemare J;Daigle F
人类特有的病原体伤寒沙门氏菌引起伤寒，这是发展中国家的一个主要公共卫生问题。其发病机制的几个方面仍然知之甚少。S.伤寒菌有14个菌毛基因簇，其中包括12个分子伴侣菌毛（stg、sth、bcf、fim、saf、sef、sta、stb、stc、std、ste和tcf）。这些菌毛在实验室条件下弱表达，只有少数被真正表征。在本研究中，所有S.伤寒分子伴侣-usher菌毛及其在发病机制中的潜在作用，如与宿主细胞的相互作用，运动性，或生物膜的形成进行了评估。所有鼠伤寒沙门伤寒菌毛在微量肉汤中表达较好。每个系统都过表达，只有菌毛基因簇没有假基因表现出一个推定的主要亚基约17 kDa的SDS-PAGE。其中六个（Fim，Saf，Sta，Stb，Std和Tcf）也通过电子显微镜显示细胞外结构。野生型菌株中菌毛缺失或将每个单独的菌毛系统添加到S.检测伤寒无菌毛株与宿主细胞的相互作用、生物膜形成和运动性。几种菌毛修饰了细菌与人细胞（THP-1和INT-407）的相互作用和生物膜形成。然而，只有Fim菌毛过表达时对运动性产生有害影响。总之，分子伴侣-引导菌毛似乎是平衡沙门氏菌不同步骤（运动、粘附、宿主入侵和持久性）的重要组成部分。伤寒发病机制。
The human-specific pathogen Salmonella enterica serovar Typhi causes typhoid, a major public health issue in developing countries. Several aspects of its pathogenesis are still poorly understood. S. Typhi possesses 14 fimbrial gene clusters including 12 chaperone-usher fimbriae (stg, sth, bcf, fim, saf, sef, sta, stb, stc, std, ste, and tcf). These fimbriae are weakly expressed in laboratory conditions and only a few are actually characterized. In this study, expression of all S. Typhi chaperone-usher fimbriae and their potential roles in pathogenesis such as interaction with host cells, motility, or biofilm formation were assessed. All S. Typhi fimbriae were better expressed in minimal broth. Each system was overexpressed and only the fimbrial gene clusters without pseudogenes demonstrated a putative major subunits of about 17 kDa on SDS-PAGE. Six of these (Fim, Saf, Sta, Stb, Std, and Tcf) also show extracellular structure by electron microscopy. The impact of fimbrial deletion in a wild-type strain or addition of each individual fimbrial system to an S. Typhi afimbrial strain were tested for interactions with host cells, biofilm formation and motility. Several fimbriae modified bacterial interactions with human cells (THP-1 and INT-407) and biofilm formation. However, only Fim fimbriae had a deleterious effect on motility when overexpressed. Overall, chaperone-usher fimbriae seem to be an important part of the balance between the different steps (motility, adhesion, host invasion and persistence) of S. Typhi pathogenesis.
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DOI：
10.1099/mic.0.2008/016998-0
发表时间：
2008-07
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Bishop A;House D;Perkins T;Baker S;Kingsley RA;Dougan G
通讯作者：
Dougan G
影响因子：
3.2
作者：
Beloin, C;Michaelis, K;Dobrindt, U
通讯作者：
Dobrindt, U
受体介导的伤寒毒素从伤寒沙门氏菌感染的细胞中出口期间。
影响因子：
30.3
作者：
Chang SJ;Song J;Galán JE
通讯作者：
Galán JE
DOI：
10.1038/nrmicro2490
发表时间：
2011-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL