Variation among natural isolates of Neurospora on small spatial scales
Variation among natural isolates of Neurospora on small spatial scales
复制标题
脉孢菌天然分离株在小空间尺度上的变异
DOI：
10.1080/15572536.2004.11833040
复制
发表时间：
2003
期刊：
影响因子：
2.8
通讯作者：
D. Natvig
中科院分区：
文献类型：
--
作者：
A. J. Powell;D. Jacobson;L. Salter;D. Natvig
虽然脉孢菌属的物种是遗传学和生物化学中研究最多的模式生物之一，但关于它们的生态学和种群生物学的基本问题仍然存在。在这项研究中，我们试图在一个小的空间尺度上澄清个体之间的关系，以评估当地的变化和殖民模式。在佛罗里达大沼泽地（1999年5月）火灾后收集了脉孢菌属的分离株，在那里，大量的菌落出现在不同的植物上，包括草和木本灌木。从两个相邻的烧焦的甘蔗茎在一个网站，并从燃烧的木本灌木在遥远的第二个网站的线性方式采样殖民地。根据杂交行为分配种属和交配类型。在两个位点，het-c和frq的变化，通过PCR产物的直接测序确定。结果表明，大量的内部和外部物种的变化，在一个小规模的，多达三个物种和六个不同的单倍型发生在一个单一的干。总共有四个物种和超过10个遗传上不同的个体（单倍型）存在于三个茎中，通常有多个个体占据同一位置。排列分析表明，个体不是随机分布的，甘蔗茎上的相邻节点更有可能被同一单倍型定殖。这表明，可见的分生孢子爆发燃烧的植物反映了大量的营养菌丝传播后，通过地下组织的主要殖民。结果还表明，相邻的菌株从一个单一的植物可以拥有不同的功能等位基因在het-c，一个观察意义的背景下，提出的作用het-c在自我识别。
Although species of Neurospora are among the most studied model organisms in genetics and biochemistry, basic questions remain with respect to their ecology and population biology. In this study, we sought to clarify relationships among individuals over a small spatial scale, toward assessing both local variation and mode of colonization. Isolates of Neurospora were collected after fires in the Florida Everglades (May 1999), where abundant colonies appeared on diverse plants, including grasses and woody shrubs. Colonies were sampled in a linear fashion from two adjacent scorched sugarcane stems at one site and from a burned woody shrub at a distant second site. Species and mating types were assigned based on crossing behavior. Variation at two loci, het-c and frq, was determined by direct sequencing of PCR products. The results demonstrated substantial within- and among-species variation on a small scale, with up to three species and six different haplotypes occurring on a single stem. In total, four species and more than 10 genetically distinct individuals (haplotypes) were present across the three stems, often with multiple individuals occupying the same position. A permutation analysis revealed that individuals were not distributed randomly and that adjacent nodes on cane stems were more likely than chance to be colonized by the same haplotype. This suggests that visible eruptions of conidia on burned plants reflect substantial vegetative mycelial spread through subsurface tissues after primary colonization. Results also revealed that adjacent isolates from a single plant can possess different functional alleles at het-c, an observation meaningful in the context of the proposed role of het-c in self recognition.
来自自然群体的脉孢菌：一项全球研究。
DOI：
10.1006/fgbi.2001.1247
发表时间：
2001
期刊：
Fungal genetics and biology : FG & B.
影响因子：
--
作者：
Turner,BC;Perkins,DD;Fairfield,A
通讯作者：
Fairfield,A
四子脉脉孢菌交配型染色体上的重组被抑制和配对异常。
影响因子：
3.3
作者：
Gallegos,A;Jacobson,DJ;Raju,NB;Skupski,MP;Natvig,DO
通讯作者：
Natvig,DO
DOI：
10.1073/pnas.91.16.7683
发表时间：
1994-08-02
影响因子：
11.1
作者：
ARONSON, BD;JOHNSON, KA;DUNLAP, JC
通讯作者：
DUNLAP, JC