Celecoxib affects estrogen sulfonation catalyzed by several human hepatic sulfotransferases, but does not stimulate 17-sulfonation in rat liver.
Celecoxib affects estrogen sulfonation catalyzed by several human hepatic sulfotransferases, but does not stimulate 17-sulfonation in rat liver.
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DOI：
10.1016/j.jsbmb.2017.05.012
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
James MO
中科院分区：
文献类型：
--
作者：
Ambadapadi S;Wang PL;Palii SP;James MO
Celecoxib is known to alter the preferred position of SULT2A1-catalyzed sulfonation of 17β-estradiol (17β-E2) and other estrogens from the 3- to the 17-position. Understanding the effects of celecoxib on estrogen sulfonation is of interest in the context of the investigational use of celecoxib to treat breast cancer. This study examined the effects on celecoxib on cytosolic sulfotransferases in human and rat liver and on SULT enzymes known to be expressed in liver. Celecoxib’s effects on the sulfonation of several steroids catalyzed by human liver cytosol were similar but not identical to those observed previously for SULT2A1. Celecoxib was shown to inhibit recombinant SULT1A1-catalyzed sulfonation of 10 nM estrone and 4 μM p-nitrophenol with IC50 values of 2.6 and 2.1 μM, respectively, but did not inhibit SULT1E1-catalyzed estrone sulfonation. In human liver cytosol, the combined effect of celecoxib and known SULT1A1 and 1E1 inhibitors, quercetin and triclosan, resulted in inhibition of 17β-E2-3-sulfonation such that the 17-sulfate became the major metabolite: this is of interest because the 17-sulfate is not readily hydrolyzed by steroid sulfatase to 17β-E2. Investigation of hepatic cytosolic steroid sulfonation in rat revealed that celecoxib did not stimulate 17β-E2 17-sulfonation in male or female rat liver as it does with human SULT2A1 and human liver cytosol, demonstrating that rat is not a useful model of this effect. In silico studies suggested that the presence of the bulky tryptophan residue in the substrate-binding site of the rat SULT2A homolog instead of glycine as in human SULT2A1 may explain this species difference.
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DOI：
10.1016/j.jsbmb.2015.05.003
发表时间：
2015-08
期刊：
The Journal of steroid biochemistry and molecular biology
影响因子：
--
作者：
Ambadapadi S;Wang PL;Palii SP;James MO
通讯作者：
James MO
影响因子：
5.8
作者：
Arnold, K;Bordoli, L;Schwede, T
通讯作者：
Schwede, T
影响因子：
1.8
作者：
Marchetti, F;De Santi, C;Pacifici, GM
通讯作者：
Pacifici, GM
DOI：
10.1016/s0960-0760(00)00040-6
发表时间：
2000-01-01
影响因子：
4.1
作者：
Chetrite, GS;Cortes-Prieto, J;Pasqualini, JR
通讯作者：
Pasqualini, JR
影响因子：
2.8
作者：
Coughtrie, MWH
通讯作者：
Coughtrie, MWH