Effect of swelling ratio of injectable hydrogel composites on chondrogenic differentiation of encapsulated rabbit marrow mesenchymal stem cells in vitro.
Effect of swelling ratio of injectable hydrogel composites on chondrogenic differentiation of encapsulated rabbit marrow mesenchymal stem cells in vitro.
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DOI：
10.1021/bm801197m
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发表时间：
2009-03-09
影响因子：
6.2
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Park, Hansoo;Guo, Xuan;Temenoff, Johnna S.;Tabata, Yasuhiko;Caplan, Arnold I.;Kasper, F. Kurtis;Mikos, Antonios G.
研究了一种可注射的、可生物降解的低聚（聚（乙二醇）富马酸酯）（OPF）和明胶微粒（MP）的水凝胶复合物作为软骨组织工程应用中的细胞和生长因子载体。本研究通过将OPF大分子单体与分子量为1000 ~ 35000的聚乙二醇（PEG）重复单元交联，制备了具有不同溶胀比的水凝胶复合材料。将兔骨髓间充质干细胞（MSCs）和负载有转化生长因子-β1（TGF-β1）的MP包封在水凝胶复合材料中，以检查在存在和不存在TGF-β1的情况下水凝胶复合材料的溶胀比对包封的兔骨髓MSCs的软骨分化的影响。水凝胶复合材料的溶胀比随着OPF大分子单体中PEG分子量的增加而增加。软骨细胞特异性基因在含有负载TGF-β1的MP的组中以更高水平表达，并且随着水凝胶复合材料的溶胀率而变化。具有分子量为35，000和10，000的PEG重复单元的OPF水凝胶复合物与负载TGF-β1的MP在第28天分别表现出II型胶原基因表达增加159 ± 95和89 ± 31倍，而具有分子量为3，000和1，000的PEG重复单元的OPF水凝胶复合物，与第0天的空白MP复合物相比，负载TGF-β1的MP复合物的II型胶原基因表达分别增加了27 ± 10和17 ± 7倍。结果表明，OPF水凝胶复合材料中的包封兔骨髓间充质干细胞的软骨分化可能受到其溶胀比的影响，从而表明OPF复合水凝胶作为控制干细胞分化的新策略的一部分的潜力。
An injectable, biodegradable hydrogel composite of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (MPs) has been investigated as a cell and growth factor carrier for cartilage tissue engineering applications. In this study, hydrogel composites with different swelling ratios were prepared by crosslinking OPF macromers with poly(ethylene glycol) (PEG) repeating units of varying molecular weights from 1,000 ~ 35,000. Rabbit marrow mesenchymal stem cells (MSCs) and MPs loaded with transforming growth factor-β1 (TGF-β1) were encapsulated in the hydrogel composites in order to examine the effect of the swelling ratio of the hydrogel composites on the chondrogenic differentiation of encapsulated rabbit marrow MSCs both in the presence and absence of TGF-β1. The swelling ratio of the hydrogel composites increased as the PEG molecular weight in the OPF macromers increased. Chondrocyte-specific genes were expressed at higher levels in groups containing TGF-β1-loaded MPs and varied with the swelling ratio of the hydrogel composites. OPF hydrogel composites with PEG repeating units of molecular weight 35,000 and 10,000 with TGF-β1-loaded MPs exhibited a 159 ± 95 and a 89 ± 31 fold increase in type II collagen gene expression at day 28, respectively, while OPF hydrogel composites with PEG repeating units of molecular weight 3,000 and 1,000 with TGF-β1-loaded MPs showed a 27 ± 10 and a 17 ± 7 fold increase in type II collagen gene expression, respectively, as compared to the composites with blank MPs at day 0. The results indicate that chondrogenic differentiation of encapsulated rabbit marrow MSCs within OPF hydrogel composites could be affected by their swelling ratio, thus suggesting the potential of OPF composite hydrogels as part of a novel strategy for controlling the differentiation of stem cells.
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影响因子：
14
作者：
Temenoff, JS;Mikos, AG
通讯作者：
Mikos, AG
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
DOI：
10.1073/pnas.142309999
发表时间：
2002-07-23
影响因子：
11.1
作者：
Kisiday, J;Jin, M;Grodzinsky, AJ
通讯作者：
Grodzinsky, AJ
影响因子：
--
作者：
Solchaga, LA;Gao, JZ;Goldberg, VM
通讯作者：
Goldberg, VM
DOI：
10.1016/j.spinee.2004.05.251
发表时间：
2005-01-01
期刊：
The spine journal : official journal of the North American Spine Society
影响因子：
--
作者：
Sobajima, Satoshi;Shimer, Adam L;Kang, James D
通讯作者：
Kang, James D