The inhibitory effect of Ulva fasciata on culturability, motility, and biofilm formation of Vibrio parahaemolyticus ATCC17802
The inhibitory effect of Ulva fasciata on culturability, motility, and biofilm formation of Vibrio parahaemolyticus ATCC17802
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石莼对副溶血弧菌 ATCC17802 培养、运动和生物膜形成的抑制作用
DOI：
10.1007/s10123-021-00165-1
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发表时间：
2021-02
影响因子：
3.1
通讯作者：
冯丽娟
中科院分区：
文献类型：
--
作者：
乔燕;贾荣;骆玉琴;冯丽娟
副溶血弧菌（V. parahaemolyticus）爆发的弧菌病是海水养殖环境中常见的致病性疾病之一。本研究通过共培养系统检测了带状石莼 (U. fasciata) 对副溶血性弧菌 ATCC17802 的可培养性、运动性和生物膜形成的抑制作用。结果表明，带状 U. fasciata 的分泌物和活体组织均可将可培养的副溶血性弧菌 ATCC17802 转化为不可培养的，共培养 3 d 后均达到 99% 以上的抑制率，且带 U. fasciata 的密度（12 g·L-1）越高，抑制作用越强。共培养3 d后，副溶血弧菌ATCC17802的抽搐行为比游泳行为更容易受到带状 U. fasciata 的影响，在不同环境因素（盐度、NO3--N和PO43--P浓度），但未发现显着相关性。对副溶血弧菌ATCC17802生物膜形成的抑制作用最大发生在12 h，抑制率在11.03-67.10%范围内，但抑制率与三种环境因素之间仍不存在显着相关性。不同的环境因素可能会诱导带状 U. fasciata 分泌不同水平的次生代谢产物，从而对副溶血性弧菌 ATCC17802 的培养、运动和生物膜形成产生不同的抑制作用。
The outbreak of vibriosis from Vibrio parahaemolyticus (V. parahaemolyticus) is one of common pathogenic diseases found in the mariculture environment. In this study, the inhibitory effect of Ulva fasciata (U. fasciata) on the culturability, motility, and biofilm formation of V. parahaemolyticus ATCC17802 was examined by co-culturing system. Results showed that both of secretion and live tissue of U. fasciata could convert culturable V. parahaemolyticus ATCC17802 to non-culturable, both reaching more than 99% of inhibition rate after 3-day co-culture, and higher density (12 g L-1) of U. fasciata exhibited stronger inhibition. The twitching behavior of V. parahaemolyticus ATCC17802 was more easily affected by U. fasciata than the swimming behavior after 3-day co-culture, with the inhibitory rates varying at the ranges of 1.70-30.29% (twitching behavior) and 10.06-44.86% (swimming behavior) under the different environmental factors (salinity, NO3--N and PO43--P concentrations), but no significant correlation was found. The greatest inhibition effect on V. parahaemolyticus ATCC17802 biofilm formation occurred at 12 h, with inhibition rates at the range of 11.03-67.10 %, while there was still no significant correlation between inhibition rate and the three environmental factors. The different environmental factors might induce U. fasciata to excrete different levels of secondary metabolites, which caused the various inhibitory effect on the cultivability, motility, and biofilm formation of V. parahaemolyticus ATCC17802.
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DOI：
--
发表时间：
2004
期刊：
--
影响因子：
--
作者：
J. Selvin;Aaron Premnath Lipton
通讯作者：
J. Selvin;Aaron Premnath Lipton
影响因子：
2
作者：
L. O. Brito;Augusto Monteiro Chagas;Elizabeth Pereira da Silva;R. Soares;W. Severi;A. Gálvez
通讯作者：
L. O. Brito;Augusto Monteiro Chagas;Elizabeth Pereira da Silva;R. Soares;W. Severi;A. Gálvez
影响因子：
1.7
作者：
S. Priyadharshini;S. Bragadeeswaran;K. Prabhu;S Sophia Ran
通讯作者：
S. Priyadharshini;S. Bragadeeswaran;K. Prabhu;S Sophia Ran
影响因子：
4.4
作者：
R. Parthasarathy;M. Chandrika;H. Rao;S. Kamalraj;C. Jayabaskaran;A. Pugazhendhi
通讯作者：
R. Parthasarathy;M. Chandrika;H. Rao;S. Kamalraj;C. Jayabaskaran;A. Pugazhendhi
影响因子：
8.9
作者：
Xiyan Mu;Gongming Shen;Ying Huang;Jianbo Luo;Lizhen Zhu;Suzhen Qi;Yingren Li;Chengju Wang
通讯作者：
Xiyan Mu;Gongming Shen;Ying Huang;Jianbo Luo;Lizhen Zhu;Suzhen Qi;Yingren Li;Chengju Wang