Tissue fixation and the effect of molecular fixatives on downstream staining procedures.
Tissue fixation and the effect of molecular fixatives on downstream staining procedures.
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DOI:
10.1016/j.ymeth.2014.01.022
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发表时间:
2014-11
期刊:
影响因子:
4.8
通讯作者:
Wilson, Beverley A.
中科院分区:
文献类型:
--
作者:
Howat, William J.;Wilson, Beverley A.
It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current “state of play” in fixation and focus on the effect fixation, and particularly the effect of the newer brand of “molecular fixatives” have on morphology, histochemistry, immunohistochemistry and RNA/DNA analysis. A methodology incorporating the creation of a fixation tissue microarray for the study of the effect of fixation on histochemistry is detailed.
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