Anti-inflammatory effects of chicanine on murine macrophage by down-regulating LPS-induced inflammatory cytokines in IκBα/MAPK/ERK signaling pathways.
Anti-inflammatory effects of chicanine on murine macrophage by down-regulating LPS-induced inflammatory cytokines in IκBα/MAPK/ERK signaling pathways.
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DOI：
10.1016/j.ejphar.2013.12.016
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发表时间：
2014-02-05
影响因子：
5
通讯作者：
Bjeldanes, Leonard F.
中科院分区：
文献类型：
--
作者：
Chen, Haixia;Sohn, Johann;Zhang, Likang;Tian, Jingge;Chen, Shuhan;Bjeldanes, Leonard F.
五味子是一种具有多种药理活性的中药。在这项研究中，研究了五味子的主要木脂素化合物之一奇卡宁对脂多糖 (LPS) 诱导的小鼠巨噬细胞（RAW 264.7 细胞）炎症反应的抑制作用。研究发现 Chicanine 具有抗炎特性，可抑制 LPS 刺激的 RAW 264.7 细胞中一氧化氮 (NO) 和前列腺素 E (2) (PGE2) 的产生以及核因子 -κB (NF-κB) 信号传导，且无细胞毒性作用。用奇卡宁处理 RAW 264.7 细胞，下调 LPS 诱导的促炎细胞因子的表达，包括 TNFα、IL-1β、MCP-1、G-CSF、环氧合酶-2 (COX-2) 和诱导型一氧化氮合酶 (iNOS)。这些抑制作用是通过阻断 p38 丝裂原激活蛋白激酶 (MAPK)、细胞外信号调节激酶 1 和 2 (ERK 1/2) 以及 IκB-α 磷酸化而发现的。这些结果表明巨噬细胞中奇卡宁的抗炎作用涉及抑制 LPS 诱导的 TLR4-IκBα/MAPK/ERK 信号通路。
Schisandra chinensis Baill is a Chinese traditional medicine with multiple pharmacological activities. In this study, chicanine, one of the major lignan compounds of Schiandra chinesis, was investigated for suppressive effects on lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (RAW 264.7 cells). Chicanine was found to have anti-infammatory properties with the inhibition of nitric oxide (NO) and Prostaglandin E (2) (PGE2) production and nuclear factor-κB (NF-κB) signaling in LPS-stimulated RAW 264.7 cells with no cytotoxic effects. Treatment of RAW 264.7 cells with chicanine down-regulated LPS-induced expression of pro-inflammatory cytokines including TNFα, IL-1β, MCP-1, G-CSF, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). These inhibitory effects were found with the blockage of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and also IκB-α phosphorylation. These results indicated that anti-inflammatory actions of chicanine in macrophages involved inhibition of LPS-induced TLR4-IκBα/MAPK/ERK signaling pathways.
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影响因子：
56.9
作者：
Johnson, GL;Lapadat, R
通讯作者：
Lapadat, R
影响因子：
3.6
作者：
Lin, Ying-Cing;Huang, Duen-Yi;Lin, Wan-Wan
通讯作者：
Lin, Wan-Wan
影响因子：
1.6
作者：
Oh, Su-Yeon;Kim, Young Hoon;Lee, Jae Kwon
通讯作者：
Lee, Jae Kwon
影响因子：
3.6
作者：
Matsunaga, Naoko;Tsuchimori, Noboru;Ii, Masayuki
通讯作者：
Ii, Masayuki
影响因子：
5.2
作者：
Wu QX;Crews MS;Draskovic M;Sohn J;Johnson TA;Tenney K;Valeriote FA;Yao XJ;Bjeldanes LF;Crews P
通讯作者：
Crews P