Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1.
Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1.
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DOI：
10.1016/j.scr.2021.102415
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发表时间：
2021-07
影响因子：
1.2
通讯作者：
Chen TY
中科院分区：
文献类型：
--
作者：
Huang PS;Wen MH;Xie X;Xu A;Lee DF;Chen TY
Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1.
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影响因子：
14.8
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