Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow.
Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow.
复制标题
DOI：
10.3346/jkms.2009.24.4.547
复制
发表时间：
2009-08
影响因子：
4.5
通讯作者：
Park YK
中科院分区：
文献类型：
--
作者：
Hwang JH;Shim SS;Seok OS;Lee HY;Woo SK;Kim BH;Song HR;Lee JK;Park YK
间充质干细胞（MSC）能够自我更新并分化成间充质组织谱系，目前正在研究其用于各种治疗应用。本研究的目的是比较来自人胎盘、脐带血 (CB) 和骨髓 (BM) 的 MSC 中细胞因子基因的表达。通过蛋白质组分析仪阵列分析比较来自 BM、CB 和胎盘（羊膜、蜕膜）的 MSC 的细胞因子表达谱。通过实时 PCR 分析了每种类型 MSC 中不同表达的细胞因子。我们评估了 36 种细胞因子。大多数类型的 MSC 具有共同的表达模式，包括 MIF (GIF、DER6)、IL-8 (CXCL8)、Serpin E1 (PAI-1)、GROα(CXCL1) 和 IL-6。然而，MCP-1 在来自骨髓和羊膜的 MSC 中都有表达。 sICAM-1 在羊膜和蜕膜 MSC 中表达。 SDF-1仅在BM MSC中表达。实时 PCR 证明了每个 MSC 中细胞因子的表达。来自骨髓、胎盘（羊膜和蜕膜）和脐带血的 MSC 表达细胞因子的方式不同。这些结果表明，来自不同组织的间充质干细胞的细胞因子诱导和信号转导是不同的。
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROα(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.
登录
查看更多内容
影响因子：
5.2
作者：
Lopez Ponte, Adriana;Marais, Emeline;Domenech, Jorge
通讯作者：
Domenech, Jorge
影响因子：
6.5
作者：
Erices, A;Conget, P;Minguell, JJ
通讯作者：
Minguell, JJ
DOI：
10.1073/pnas.95.16.9448
发表时间：
1998-08-04
影响因子：
11.1
作者：
Ma, Q;Jones, D;Springer, TA
通讯作者：
Springer, TA
影响因子：
5.2
作者：
Ji, JF;He, BP;Tay, SSW
通讯作者：
Tay, SSW
影响因子：
5.2
作者：
in't Anker, PS;Scherjon, SA;Kanhai, HHH
通讯作者：
Kanhai, HHH