Complete genome sequence and comparative genomic analysis of Mycobacterium massiliense JCM 15300 in the Mycobacterium abscessus group reveal a conserved genomic island MmGI-1 related to putative lipid metabolism.
Complete genome sequence and comparative genomic analysis of Mycobacterium massiliense JCM 15300 in the Mycobacterium abscessus group reveal a conserved genomic island MmGI-1 related to putative lipid metabolism.
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腹部分枝杆菌组中的分枝杆菌JCM 15300的完整基因组序列和比较基因组分析显示，与推定的脂质代谢相关的保守基因组岛MMGI-1。
DOI：
10.1371/journal.pone.0114848
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kuroda M
中科院分区：
文献类型：
--
作者：
Sekizuka T;Kai M;Nakanaga K;Nakata N;Kazumi Y;Maeda S;Makino M;Hoshino Y;Kuroda M
分枝杆菌群亚种，如M. M. tagliense，M.严格意义上的刺蛾和M.博莱氏菌是一种存在于土壤、水体等生态环境中的环境生物，已从呼吸道感染、皮肤软组织感染、整容手术后感染中分离到。为了确定M.对M. JCM 15300（对应于CCUG 48898）。对分枝杆菌属进行比较基因组分析。在M.刺蛾组亚种，表明其他的β-氧化相关基因，特别是哺乳动物细胞进入（mce）操纵子位于基因组岛上，M. MmGI-1基因组岛1（MmGI-1），在M.阿格里真托。此外，还发现了与厌氧呼吸系统相关的基因和分枝菌酸环丙烷合成酶相关的基因。阿格里真托。日本分离株M.利什曼原虫也经常拥有MmGI-1（14/44，约32%）和三个独特的保守区域（26/44;约60%，34/44;约77%和40/44;约91%），以及其他国家（马来西亚，法国，英国和美国）的分离株。高度保守的基因组岛MmGI-1可能在高生长潜力中发挥重要作用，具有额外的脂质代谢、在环境中生存的额外因素或合成复杂的膜相关脂质。MmGI-1的ORF与近缘M.禽复合体（MAC），表明MmGI-1可能发生了水平基因转移或遗传重组事件。马可和马克。
Mycobacterium abscessus group subsp., such as M. massiliense, M. abscessus sensu stricto and M. bolletii, are an environmental organism found in soil, water and other ecological niches, and have been isolated from respiratory tract infection, skin and soft tissue infection, postoperative infection of cosmetic surgery. To determine the unique genetic feature of M. massiliense, we sequenced the complete genome of M. massiliense type strain JCM 15300 (corresponding to CCUG 48898). Comparative genomic analysis was performed among Mycobacterium spp. and among M. abscessus group subspp., showing that additional ß-oxidation-related genes and, notably, the mammalian cell entry (mce) operon were located on a genomic island, M. massiliense Genomic Island 1 (MmGI-1), in M. massiliense. In addition, putative anaerobic respiration system-related genes and additional mycolic acid cyclopropane synthetase-related genes were found uniquely in M. massiliense. Japanese isolates of M. massiliense also frequently possess the MmGI-1 (14/44, approximately 32%) and three unique conserved regions (26/44; approximately 60%, 34/44; approximately 77% and 40/44; approximately 91%), as well as isolates of other countries (Malaysia, France, United Kingdom and United States). The well-conserved genomic island MmGI-1 may play an important role in high growth potential with additional lipid metabolism, extra factors for survival in the environment or synthesis of complex membrane-associated lipids. ORFs on MmGI-1 showed similarities to ORFs of phylogenetically distant M. avium complex (MAC), suggesting that horizontal gene transfer or genetic recombination events might have occurred within MmGI-1 among M. massiliense and MAC.
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DOI：
10.1093/bioinformatics/btq224
发表时间：
2010-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Katoh K;Toh H
通讯作者：
Toh H
影响因子：
4.2
作者：
Kim SH;Harzman C;Davis JK;Hutcheson R;Broderick JB;Marsh TL;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.7
作者：
Banerjee R;Vats P;Dahale S;Kasibhatla SM;Joshi R
通讯作者：
Joshi R
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
分枝杆菌基因组数据库。
影响因子：
1.9
作者：
Joseph J;Rajendran V;Hassan S;Kumar V
通讯作者：
Kumar V