Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids.
Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids.
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DOI：
10.3390/ijms231911406
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发表时间：
2022-09-27
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
牡丹杂交种是以牡丹组和牡丹组分别为父本和母本的杂交品种。因此，这些杂交种具有由父本引进的草本茎，具有更好的观赏价值。虽然它们的双亲都是二倍体，但伊藤杂交后代是三倍体。此外，它们染色体的亲本起源还没有得到广泛的研究。本研究系统地分析了伊藤杂交种的基因组大小、倍性和核型，并与亲本类群进行了比较。伊藤杂交种的单倍体基因组大小虽然不同，但与亲本差异不显著。然而，两个亲本分类群的品种大小与野生种有显著差异，这可能是由于人工选择引起的基因组重排所致。进一步的核型分析、相关分析和等级聚类分析都不能确定伊藤杂交种染色体的亲本来源。通过基因组和荧光原位杂交（GISH和FISH）验证，伊藤杂交种的3对染色体中，2对来自父本，1对来自母本。前两组中的一组来自野生物种，另一组来自栽培品种。与野生牡丹相比，栽培牡丹的基因组庞大而复杂，因此GISH无法标记牡丹的染色体。同时，基于5S rdna的FISH首次应用于芍药，可用于倍性评价。本研究为伊藤杂交资源的利用提供了新的思路。
Itoh hybrids are intersectional hybrids in Paeonia L. with sect. Moutan and sect. Paeonia as paternal and maternal parents, respectively. Therefore, these hybrids have herbaceous stems with improved ornamental value introduced by the paternal parent. Although both of their parents are diploids, Itoh hybrids are triploids. Moreover, the parental origin of their chromosomes has not been extensively studied. This study systematically analyzed the genome size, ploidy, and karyotype of Itoh hybrids and compared them with their parental taxa. Although the monoploid genome size of Itoh hybrids was different, it was not significantly different from that of the parents. However, the size of varieties in the two parental taxa was significantly different from the wild species, probably due to genome rearrangements caused by artificial selection. Further karyotype analysis, correlation analysis, and hierarchical clustering could not identify the parental origin of chromosomes in Itoh hybrids. Verification through genomic and fluorescence in situ hybridization (GISH and FISH) suggested that for the three sets of chromosomes in Itoh hybrids, two were from the paternal parent, and one was from the maternal parent. One of the first two sets was from wild species, and the other from a cultivated variety. GISH could not label the chromosomes of cultivated peonies from the sect. Moutan, probably due to the huge and complex genomes compared with the wild species. Meanwhile, 5S rDNA-based FISH was first applied in Paeonia, which may be used for ploidy assessment. This work may give insights into the utilization of Itoh hybrid resources.
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DOI：
10.1016/j.ppees.2015.11.001
发表时间：
2016-02-01
影响因子：
3.6
作者：
Baltisberger, Matthias;Hoerandl, Elvira
通讯作者：
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影响因子：
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影响因子：
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Modarressi, M. H.
影响因子：
1.4
作者：
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通讯作者：
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作者：
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