Genome-Wide Identification and Expression Profiling of Tomato Hsp20 Gene Family in Response to Biotic and Abiotic Stresses
Genome-Wide Identification and Expression Profiling of Tomato Hsp20 Gene Family in Response to Biotic and Abiotic Stresses
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番茄 Hsp20 基因家族响应生物和非生物胁迫的全基因组鉴定和表达谱
DOI：
10.3389/fpls.2016.01215
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发表时间：
2016-08
影响因子：
5.6
通讯作者：
Wan, Hongjian
中科院分区：
文献类型：
--
作者：
Zhou, Guozhi;Yao, Zhuping;Yang, Yuejian;Wan, Hongjian
Hsp20基因参与植物对包括热休克在内的环境胁迫的反应，在植物的生长发育中也起着至关重要的作用。它们代表了植物中最丰富的小热休克蛋白（sHsps），但对世界上重要的蔬菜作物番茄（Solanum lycopersicum）中的这一家族知之甚少。本文从基因结构、染色体定位、系统发育关系和表达谱等方面对番茄热休克蛋白20 （SlHsp20）基因家族进行了分析。利用基于生物信息学的方法，我们在番茄中鉴定了至少42个假定的SlHsp20基因。序列分析显示，大部分SlHsp20基因不含内含子或内含子长度较短。染色体定位表明，臂间和染色体内复制事件对SlHsp20基因的扩增有显著的促进作用。对来自番茄和其他植物物种的Hsp20基因进行系统发育树分析，发现SlHsp20基因可分为13个亚科，表明这些基因可能有一个共同的祖先，在单双子叶分裂之前产生了不同的亚科。此外，利用RNA-seq技术对栽培番茄和野生近缘茄（Solanum pimpinellifolium）在不同组织和发育阶段的表达进行分析，发现这些基因中的大部分（83%）在至少一个基因型的至少一个阶段表达。在42个基因中，有4个基因在几乎所有组织中都有组成性表达，这意味着这些基因在正常生长条件下可能在番茄细胞中具有特定的管家功能。两个SlHsp20基因在栽培番茄和细穗花的营养器官（叶和根）和生殖器官（花芽和花）中表现出差异表达水平，表明在驯化过程中，种间多样化以实现功能专门化。基于全基因组微阵列分析，我们发现SlHsp20基因的转录水平可以被热、干旱、盐、番茄灰孢菌和番茄斑点枯萎病毒（TSWV）等非生物和生物胁迫大量诱导，这表明它们在介导番茄植物对环境胁迫的反应中具有潜在的作用。综上所述，这些结果为进一步阐明Hsp20基因家族的进化关系和SlHsp20基因家族的功能表征提供了有价值的信息。
The Hsp20 genes are involved in the response of plants to environment stresses including heat shock and also play a vital role in plant growth and development. They represent the most abundant small heat shock proteins (sHsps) in plants, but little is known about this family in tomato (Solanum lycopersicum), an important vegetable crop in the world. Here, we characterized heat shock protein 20 (SlHsp20) gene family in tomato through integration of gene structure, chromosome location, phylogenetic relationship, and expression profile. Using bioinformatics-based methods, we identified at least 42 putative SlHsp20 genes in tomato. Sequence analysis revealed that most of SlHsp20 genes possessed no intron or a relatively short intron in length. Chromosome mapping indicated that inter-arm and intra-chromosome duplication events contributed remarkably to the expansion of SlHsp20 genes. Phylogentic tree of Hsp20 genes from tomato and other plant species revealed that SlHsp20 genes were grouped into 13 subfamilies, indicating that these genes may have a common ancestor that generated diverse subfamilies prior to the mono-dicot split. In addition, expression analysis using RNA-seq in various tissues and developmental stages of cultivated tomato and the wild relative Solanum pimpinellifolium revealed that most of these genes (83%) were expressed in at least one stage from at least one genotype. Out of 42 genes, 4 genes were expressed constitutively in almost all the tissues analyzed, implying that these genes might have specific housekeeping function in tomato cell under normal growth conditions. Two SlHsp20 genes displayed differential expression levels between cultivated tomato and S. pimpinellifolium in vegetative (leaf and root) and reproductive organs (floral bud and flower), suggesting inter-species diversification for functional specialization during the process of domestication. Based on genome-wide microarray analysis, we showed that the transcript levels of SlHsp20 genes could be induced profusely by abiotic and biotic stresses such as heat, drought, salt, Botrytis cinerea, and Tomato Spotted Wilt Virus (TSWV), indicating their potential roles in mediating the response of tomato plants to environment stresses. In conclusion, these results provide valuable information for elucidating the evolutionary relationship of Hsp20 gene family and functional characterization of the SlHsp20 gene family in the future.
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辣椒CaHsp20基因家族的全基因组分析：热胁迫下的综合序列和表达谱分析
影响因子：
5.6
作者：
Guo M;Liu JH;Lu JP;Zhai YF;Wang H;Gong ZH;Wang SB;Lu MH
通讯作者：
Lu MH
DOI：
10.1002/9781119432401.ch9
发表时间：
2020-02
期刊：
Heat Stress Tolerance in Plants
影响因子：
--
作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
通讯作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
1.5
作者：
P. Nautiyal;M. Shono
通讯作者：
P. Nautiyal;M. Shono
影响因子：
--
作者：
R. V. Montfort;C. Slingsby;Elizabeth Vierlingt
通讯作者：
R. V. Montfort;C. Slingsby;Elizabeth Vierlingt