Preprogrammed, parallel on-chip immunoassay using system-level capillarity control.
Preprogrammed, parallel on-chip immunoassay using system-level capillarity control.
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DOI：
10.1021/ac401292d
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发表时间：
2013-07-16
影响因子：
7.4
通讯作者：
Kurabayashi, Katsuo
中科院分区：
文献类型：
--
作者：
Kim, Sung-Jin;Paczesny, Sophie;Takayama, Shuichi;Kurabayashi, Katsuo
传统多孔板的全手工使用使得基于酶联免疫吸附试验(ELISA)的免疫分析非常耗时和劳动密集型。在这里，我们提出了一种毛细管驱动的芯片上免疫分析，它以一种廉价的设置大大节省了时间和劳动力。我们的免疫分析过程首先将多个溶液移液到构建在微流控设备芯片上的多个孔中。随后，毛细管以纯被动和预先编程的方式，自发地将多个样品溶液和常用试剂溶液传输到芯片上指定的检测通道中。我们的设备通过协调芯片上无源组件的功能，在30分钟内实现了涉及4个样本和6个试剂溶液的毛细管驱动免疫分析。值得注意的是，我们的免疫分析技术将移液过程的总数减少了~5倍，而在多孔板上的分析(48比10)。这项检测技术使我们能够定量C反应蛋白和致瘤性抑制因子2的浓度，检测下限分别为8 PM和90 PM。该装置应可用于在资源有限的护理场所进行复杂的、并行的生化微流控处理。
Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ~5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources.
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影响因子：
2.7
作者：
Cho, SK;Moon, HJ;Kim, CJ
通讯作者：
Kim, CJ
影响因子：
7.4
作者：
Hossain, S. M. Zakir;Brennan, John D.
通讯作者：
Brennan, John D.
影响因子：
6.1
作者：
Lewis, Gregory G.;DiTucci, Matthew J.;Phillips, Scott T.
通讯作者：
Phillips, Scott T.
影响因子：
6.1
作者：
Chen, Hong;Cogswell, Jeremy;Faghri, Mohammad
通讯作者：
Faghri, Mohammad
蜡印硝化纤维素膜纸基微流控的制备和表征
影响因子：
7.4
作者：
Lu, Yao;Shi, Weiwei;Lin, Bingcheng
通讯作者：
Lin, Bingcheng