Functional analysis of seven regulators of G protein signaling (RGSs) in the nematode-trapping fungus Arthrobotrys oligospora.
Functional analysis of seven regulators of G protein signaling (RGSs) in the nematode-trapping fungus Arthrobotrys oligospora.
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在线虫捕获真菌性真菌寡孢子中，G蛋白信号传导（RGSS）的七个调节剂的功能分析。
DOI：
10.1080/21505594.2021.1948667
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Ma N;Zhao Y;Wang Y;Yang L;Li D;Yang J;Jiang K;Zhang KQ;Yang J
G蛋白信号转导调节因子（Regulators of G protein signaling，RGSs）是一种负性调节G蛋白信号转导的蛋白质。在这项研究中，七个假定的RGSs的特点是在线虫捕捉（NT）真菌，少孢节孢菌。删除Rgs基因显著增加胞内cAMP水平，并导致菌丝生长，抗逆性，分生孢子，陷阱形成和杀线虫活性的缺陷。特别地，ΔAoFlbA突变体不能产生分生孢子和陷阱。转录组学分析表明，与WT相比，ΔAoFlbA突变体中氨基酸代谢和生物合成过程显著富集。有趣的是，Gas1家族基因在A. oligospora和其他NT真菌产生粘附陷阱，并在A.寡孢菌。两个Gas1基因的破坏导致有缺陷的分生孢子，陷阱的形成，和致病性。我们的结果表明，RGSs在调节A.寡孢菌菌丝生长、发育和致病性。此外，AoFlbA是一个突出的成员，可能通过调节氨基酸代谢和生物合成，需要分生孢子和陷阱的形成。我们的研究结果为阐明NT真菌营养生长、生活方式转变和致病性的信号机制提供了基础。
Regulators of G protein signaling (RGSs) are proteins that negatively regulate G protein signal transduction. In this study, seven putative RGSs were characterized in the nematode-trapping (NT) fungus, Arthrobotrys oligospora. Deleting Rgs genes significantly increased intracellular cAMP levels, and caused defects in mycelia growth, stress resistance, conidiation, trap formation, and nematocidal activity. In particular, the ΔAoFlbA mutant was unable to produce conidia and traps. Transcriptomic analysis showed that amino acid metabolic and biosynthetic processes were significantly enriched in the ΔAoFlbA mutant compared to WT. Interestingly, Gas1 family genes are significantly expanded in A. oligospora and other NT fungi that produce adhesive traps, and are differentially expressed during trap formation in A. oligospora. Disruption of two Gas1 genes resulted in defective conidiation, trap formation, and pathogenicity. Our results indicate that RGSs play pleiotropic roles in regulating A. oligospora mycelial growth, development, and pathogenicity. Further, AoFlbA is a prominent member and required for conidiation and trap formation, possibly by regulating amino acid metabolism and biosynthesis. Our results provide a basis for elucidating the signaling mechanism of vegetative growth, lifestyle transition, and pathogenicity in NT fungi.
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作者：
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