Effects of partially and more highly purified platelet-derived growth factor preparations on luteinizing hormone receptor induction in granulosa cell cultures.
Effects of partially and more highly purified platelet-derived growth factor preparations on luteinizing hormone receptor induction in granulosa cell cultures.
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部分和更高纯度的血小板衍生生长因子制剂对颗粒细胞培养物中黄体生成素受体诱导的影响。
DOI：
10.1095/biolreprod30.3.603
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发表时间：
1984
影响因子：
3.6
通讯作者：
Schomberg,DW
中科院分区：
文献类型：
--
作者：
Mondschein,JS;Schomberg,DW
在培养的未成熟的、己烯雌酚致敏的大鼠颗粒细胞中，研究了部分和更高纯度的血小板衍生生长因子（PDGF）制剂对促卵泡激素（FSH）和霍乱毒素（CTX）诱导黄体生成素（LH）受体的影响。通过羧甲基葡聚糖C-50色谱法（CMS-PDGF）制备的部分纯化的PDGF和通过汽巴克隆蓝琼脂糖色谱法（BS-PDGF）进一步纯化的更高度纯化的PDGF在无血清和含血清培养基中增强FSH依赖性LH受体诱导。BS-PDGF比CMS-PDGF更有效，并且在无血清培养基中比CMS-PDGF相对更有效，而在含血清培养基中比CMS-PDGF有效性低。CMS-PDGF和BS-PDGF增强CTX在含血清培养基中对LH受体的诱导，但达到的水平显著低于FSH和CMS-或BS-PDGF达到的水平。BS-PDGF不增强CTX在无血清培养基中的诱导。结果表明，PDGF增强LH受体诱导的作用是复杂的，并可能代表PDGF制剂的几种成分的作用。这些发现也可能为FSH作用的一个独立于cAMP的成分提供间接证据。
The effects of partially and more highly purified platelet-derived growth factor (PDGF) preparations on luteinizing hormone (LH) receptor induction by follicle-stimulating hormone (FSH) and cholera toxin (CTX) were studied in cultured granulosa cells from immature, diethylstilbestrol-primed rats. Partially purified PDGF, prepared by carboxymethyl Sephadex C-50 chromatography (CMS-PDGF), and more highly purified PDGF, further purified by Cibacron Blue Sepharose chromatography (BS-PDGF), enhance FSH-dependent LH receptor induction in serum-free and in serum-containing medium. BS-PDGF is more potent than CMS-PDGF, and is relatively more efficacious in serum-free and less efficacious in serum-containing medium than CMS-PDGF. CMS-PDGF and BS-PDGF enhance LH receptor induction by CTX in serum-containing medium, but levels achieved are significantly less than those attained with FSH and CMS- or BS-PDGF. BS-PDGF does not enhance induction by CTX in serum-free medium. The results suggest that the action of PDGF to enhance LH receptor induction is complex and may represent actions of several components of PDGF preparations. These findings may also provide indirect evidence for a component of FSH action which is independent of cAMP.
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DOI：
10.1073/pnas.76.4.1809
发表时间：
1979-01-01
影响因子：
11.1
作者：
ANTONIADES, HN;SCHER, CD;STILES, CD
通讯作者：
STILES, CD
在培养的颗粒细胞中通过腺苷 3,5-一磷酸诱导黄体生成激素受体。
影响因子：
4.8
作者：
M. Knecht;K. Catt
通讯作者：
K. Catt
表皮生长因子和血小板衍生生长因子调节 cAMP 介导的卵巢颗粒细胞分化。
影响因子：
4.8
作者：
M. Knecht;Kevin;Catt
通讯作者：
Catt
血小板衍生生长因子可增强卵泡刺激素和血清对颗粒细胞黄体生成素受体的诱导。
影响因子：
4.8
作者：
Mondschein,JS;Schomberg,DW
通讯作者：
Schomberg,DW