Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia.
Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia.
复制标题
DOI：
10.1186/1471-2180-11-51
复制
发表时间：
2011-03-08
期刊：
影响因子：
4.2
通讯作者：
Williams P
中科院分区：
文献类型：
--
作者：
Chan KG;Atkinson S;Mathee K;Sam CK;Chhabra SR;Cámara M;Koh CL;Williams P
细胞间通讯（群体感应（QS））在群体水平上协调细菌行为。因此，自然多物种群落的行为可能至少部分取决于共存的 QS 和群体淬灭 (QQ) 活动。在这里，我们试图通过调查与生长在马来西亚热带雨林的生姜（Zingiber officinale）根际相关的细菌群落来发现新的 N-酰基高丝氨酸内酯（AHL）依赖性 QS 和 QQ 菌株。通过使用含有 N-(3-氧代己酰基)高丝氨酸内酯 (3-oxo-C6-HSL) 作为唯一碳源和氮源的基础生长培养基，生姜根际相关细菌富集了具有 AHL 降解能力的菌株。鉴定出属于不动杆菌属 (GG2)、伯克霍尔德杆菌属 (GG4) 和克雷伯氏菌属 (Se14) 的三种分离株，并选择用于进一步研究。菌株 GG2 和 Se14 通过内酯分解表现出最广谱的 AHL 降解活性，而 GG4 将 3-氧代-AHL 还原为相应的 3-羟基化合物。在 GG2 和 GG4 中，发现 QQ 与 AHL 依赖性 QS 共存，并且 GG2 可以使自生和外源提供的 AHL 失活。 GG2、GG4 和 Se14 均能够减弱人类和植物病原体中毒力因子的产生。总的来说，我们的数据表明，产生和降解多种 AHL 的生姜根际细菌可能在确定多微生物群落的 QS 依赖性表型中发挥集体作用。
Cell-to-cell communication (quorum sensing (QS)) co-ordinates bacterial behaviour at a population level. Consequently the behaviour of a natural multi-species community is likely to depend at least in part on co-existing QS and quorum quenching (QQ) activities. Here we sought to discover novel N-acylhomoserine lactone (AHL)-dependent QS and QQ strains by investigating a bacterial community associated with the rhizosphere of ginger (Zingiber officinale) growing in the Malaysian rainforest. By using a basal growth medium containing N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6-HSL) as the sole source of carbon and nitrogen, the ginger rhizosphere associated bacteria were enriched for strains with AHL-degrading capabilities. Three isolates belonging to the genera Acinetobacter (GG2), Burkholderia (GG4) and Klebsiella (Se14) were identified and selected for further study. Strains GG2 and Se14 exhibited the broadest spectrum of AHL-degrading activities via lactonolysis while GG4 reduced 3-oxo-AHLs to the corresponding 3-hydroxy compounds. In GG2 and GG4, QQ was found to co-exist with AHL-dependent QS and GG2 was shown to inactivate both self-generated and exogenously supplied AHLs. GG2, GG4 and Se14 were each able to attenuate virulence factor production in both human and plant pathogens. Collectively our data show that ginger rhizosphere bacteria which make and degrade a wide range of AHLs are likely to play a collective role in determining the QS-dependent phenotype of a polymicrobial community.
登录
查看更多内容
影响因子：
3.2
作者：
Niu, Chen;Clemmer, Katy M.;Rather, Philip N.
通讯作者：
Rather, Philip N.
DOI：
10.1007/s10482-010-9438-0
发表时间：
2010-10-01
影响因子：
2.6
作者：
Chan, Kok-Gan;Wong, Cheng-Siang;Koh, Chong-Lek
通讯作者：
Koh, Chong-Lek
影响因子：
3.2
作者：
Gray, KM;Pearson, JP;Greenberg, EP
通讯作者：
Greenberg, EP
影响因子：
2.8
作者：
Kang, BR;Lee, JH;Kim, YC
通讯作者：
Kim, YC
DOI：
10.1099/00221287-146-10-2469
发表时间：
2000-10-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Laue, RE;Jiang, Y;Williams, P
通讯作者：
Williams, P