Effect of molecular structure of cationic surfactants on biophysical interactions of surfactant-modified nanoparticles with a model membrane and cellular uptake.
Effect of molecular structure of cationic surfactants on biophysical interactions of surfactant-modified nanoparticles with a model membrane and cellular uptake.
复制标题
DOI：
10.1021/la803361y
复制
发表时间：
2009-02-17
期刊：
影响因子：
--
通讯作者：
Labhasetwar V
中科院分区：
文献类型：
--
作者：
Peetla C;Labhasetwar V
本研究的目的是验证一种假设，即纳米粒子(NP)界面上的阳离子表面活性剂的分子结构影响NPs与模型膜的生物物理相互作用和细胞对NPs的摄取。用阳离子表面活性剂对不含表面活性剂的聚苯乙烯纳米粒子(130 Nm)进行了改性。这些表面活性剂要么是双链的(二十二烷基二甲基溴化铵[DMAB])，要么是单链的(十六烷基三甲基溴化铵[CTAB]和十二烷基三甲基溴化铵[DTAB])，后两者具有不同的疏水链长。用朗缪尔膜天平研究了这些表面活性剂修饰的纳米颗粒与内皮细胞模型膜(EMM)的生物物理相互作用。分析了膜表面压力(SP)随时间的变化以及膜脂混合物在NPs存在下的压缩等温线(π-A)。用原子力显微镜(AFM)对转移到合适衬底上的电磁记忆膜(LS)进行了成像，并对图像进行了分析，以确定NP-EMM相互作用的机制。与CTAB和DTAB修饰的NPs相比，DMAB修饰的NPs有更大的SP增加，并向更高的平均分子面积(MMA)移动，这表明DMAB修饰的NPs与EMM有更强的相互作用。然而，AFM物相和高度图像分析表明，DMAB和CTAB修饰的NPs都与EMM相互作用，但通过不同的机制：DMAB修饰的NPs穿透EMM，从而解释了SP的增加，而CTAB修饰的NPs锚定在EMM的凝聚脂结构域上，因此没有引起SP的任何显著变化。人脐静脉内皮细胞对DMAB和CTAB修饰的NPs的摄取高于DTAB修饰或未修饰的NPs。我们得出的结论是：(I)纳米粒子表面的双链和单链阳离子表面活性剂与模型膜具有不同的相互作用机制，(Ii)与模型膜具有更强的生物物理相互作用的纳米粒子也表现出更强的细胞摄取能力。因此，纳米粒子与模型膜的生物物理相互作用可以有效地用于开发具有优化表面性质的纳米载体，用于药物输送和成像应用。
The aim of this study was to test the hypothesis that the molecular structure of cationic surfactants at the nanoparticle (NP)-interface influences the biophysical interactions of NPs with a model membrane and cellular uptake of NPs. Polystyrene NPs (surfactant free, 130 nm) were modified with cationic surfactants. These surfactants were of either dichained (didodecyldimethylammonium bromide [DMAB]) or single chained (cetyltrimethylammonium bromide [CTAB] and dodecyltrimethylammonium bromide [DTAB]) forms, the latter two with different hydrophobic chain lengths. Biophysical interactions of these surfactant-modified NPs with an endothelial cell model membrane (EMM) were studied using a Langmuir film balance. Changes in surface pressure (SP) of EMM as a function of time following interaction with NPs and in the compression isotherm (π - A) of the lipid mixture of EMM in the presence of NPs were analyzed. Langmuir-Schaeffer (LS) films, which are EMMs that have been transferred onto a suitable substrate, were imaged by atomic force microscopy (AFM), and the images were analyzed to determine the mechanisms of the NP-EMM interaction. DMAB-modified NPs showed a greater increase in SP and a shift towards higher mean molecular area (mmA) than CTAB- and DTAB-modified NPs, indicating stronger interactions of DMAB-modified NPs with the EMM. However, analysis of the AFM phase and height images of the LS films revealed that both DMAB- and CTAB-modified NPs interacted with the EMM but via different mechanisms: DMAB-modified NPs penetrated the EMM, thus explaining the increase in SP, whereas CTAB-modified NPs anchored onto the EMM's condensed lipid domains, and hence did not cause any significant change in SP. Human umbilical vein endothelial cells showed greater uptake of DMAB- and CTAB-modified NPs than of DTAB-modified or unmodified NPs. We conclude that (i) the dichained and single-chained cationic surfactants on NPs have different mechanisms of interaction with the model membrane and (ii) NPs that demonstrate greater biophysical interactions with the membrane also show greater cellular uptake. Biophysical interactions of NPs with a model membrane thus could be effectively used for developing nanocarriers with optimized surface properties for drug delivery and imaging applications.
登录
查看更多内容
影响因子：
14
作者：
Vasir, Jaspreet K.;Labhasetwar, Vinod
通讯作者：
Labhasetwar, Vinod
影响因子：
--
作者：
Kumar, MNVR;Mohapatra, SS;Lehr, CM
通讯作者：
Lehr, CM
影响因子：
2.4
作者：
Peetla, Chiranjeevi;Graf, Karlheinz;Kressler, Joerg
通讯作者：
Kressler, Joerg
影响因子：
4.9
作者：
Peetla, Chiranjeevi;Labhasetwar, Vinod
通讯作者：
Labhasetwar, Vinod
影响因子：
4.5
作者：
Lockman, PR;Koziara, JM;Allen, DD
通讯作者：
Allen, DD