Activation of NMDA receptors in the brainstem, rostral ventromedial medulla, and nucleus reticularis gigantocellularis mediates mechanical hyperalgesia produced by repeated intramuscular injections of acidic saline in rats.
Activation of NMDA receptors in the brainstem, rostral ventromedial medulla, and nucleus reticularis gigantocellularis mediates mechanical hyperalgesia produced by repeated intramuscular injections of acidic saline in rats.
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DOI：
10.1016/j.jpain.2009.08.006
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Sluka KA
中科院分区：
文献类型：
--
作者：
Da Silva LF;Desantana JM;Sluka KA
重复注射酸性生理盐水到腓肠肌诱导肌肉和皮肤过敏。我们以前已经表明，局部麻醉剂微量注射到头端腹内侧延髓（RVM）或网状神经细胞核（NGC）逆转这种肌肉和皮肤的超敏反应。尽管先前的研究表明RVM中的NMDA受体在介导内脏和炎性超敏反应中起着明确的作用，但NMDA受体在NGC或非炎性肌肉疼痛中的作用尚不清楚。因此，本研究评价了重复肌肉注射酸性盐水诱导的肌肉和皮肤超敏反应中RVM和NGC中NMDA受体的参与。重复肌内注射酸性生理盐水，间隔5天，导致在第二次注射后24小时，在所有组的爪和肌肉的缩足阈值的双边下降。RVM内微量注射NMDA受体拮抗剂可逆转肌肉和皮肤的超敏反应。然而，在NGC中微量注射NMDA受体拮抗剂只能逆转皮肤，但不能逆转肌肉超敏反应。这些结果表明，在RVM的NMDA受体介导的肌肉和皮肤的超敏反应，但在NGC介导的皮肤过敏性肌肉损伤后。
Repeated injections of acidic saline into the gastrocnemius muscle induced both muscle and cutaneous hypersensitivity. We have previously shown that microinjection of local anesthetic into either the rostral ventromedial medulla (RVM) or the nucleus reticularis gigantocellularis (NGC) reverses this muscle and cutaneous hypersensitivity. Although prior studies show that NMDA receptors in the RVM play a clear role in mediating visceral and inflammatory hypersensitivity, the role of NMDA receptors in the NGC, or in non-inflammatory muscle pain is unclear. Therefore, the present study evaluated involvement of the NMDA receptors in the RVM and NGC in muscle and cutaneous hypersensitivity induced by repeated intramuscular injections of acidic saline. Repeated intramuscular injections of acidic saline, 5 days apart, resulted in a bilateral decrease in the withdrawal thresholds of the paw and muscle in all groups 24 h after the second injection. Microinjection of NMDA receptor antagonists into the RVM reversed both the muscle and cutaneous hypersensitivity. However, microinjection of NMDA receptor antagonists into the NGC only reversed cutaneous, but not muscle hypersensitivity. These results suggest that NMDA receptors in the RVM mediate both muscle and cutaneous hypersensitivity, but those in the NGC mediated only cutaneous hypersensitivity after muscle insult.
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