Coherent anti-Stokes Raman scattering (CARS) spectroscopy in Caenorhabditis elegans and Globodera pallida: evidence for an ivermectin-activated decrease in lipid stores.
Coherent anti-Stokes Raman scattering (CARS) spectroscopy in Caenorhabditis elegans and Globodera pallida: evidence for an ivermectin-activated decrease in lipid stores.
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DOI：
10.1002/ps.4707
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发表时间：
2017-12
影响因子：
4.1
通讯作者：
Mahajan S
中科院分区：
文献类型：
--
作者：
Smus JP;Ludlow E;Dallière N;Luedtke S;Monfort T;Lilley C;Urwin P;Walker RJ;O'Connor V;Holden-Dye L;Mahajan S
大环内酯可以说是对寄生线虫有效的最成功的化学类别。在这里，我们研究了大环内酯伊维菌素对植物寄生线虫Globodera pallida脂质稳态的影响，并提供了新的见解，其作用方式。采用一种称为相干反斯托克斯拉曼散射（汽车）光谱的非侵入性、非破坏性、无标记和化学选择性技术研究G.苍白球我们使用自由生活的线虫秀丽隐杆线虫优化了该方案，然后使用汽车来定量G.苍白球。这揭示了在孵化后24小时内J2 s后部区域的脂质储存浓度在28天的过程中降低到不可检测的水平。我们测试了伊维菌素对J2活力和脂质储存的影响。伊维菌素在24 h内使J2麻痹。与直觉相反，在相同的时间过程中，伊维菌素增加了J2脂质的消耗速率，这表明在伊维菌素处理的J2中，运动和代谢率的能量需求之间存在脱节。预计这种脂质储存的减少将对J2感染潜力产生负面影响。这些数据表明，大环内酯作为种子处理剂的益处可能受到涉及神经肌肉抑制和脂质代谢加速的多层次效应的支持。版权所有© 2017作者.有害生物管理科学由John Wiley & Sons Ltd代表化学工业协会出版。
Macrocyclic lactones are arguably the most successful chemical class with efficacy against parasitic nematodes. Here we investigated the effect of the macrocyclic lactone ivermectin on lipid homeostasis in the plant parasitic nematode Globodera pallida and provide new insight into its mode of action. A non‐invasive, non‐destructive, label‐free and chemically selective technique called Coherent anti‐Stokes Raman scattering (CARS) spectroscopy was used to study lipid stores in G. pallida. We optimised the protocol using the free‐living nematode Caenorhabditis elegans and then used CARS to quantify lipid stores in the pre‐parasitic, non‐feeding J2 stage of G. pallida. This revealed a concentration of lipid stores in the posterior region of J2 s within 24 h of hatching which decreased to undetectable levels over the course of 28 days. We tested the effect of ivermectin on J2 viability and lipid stores. Within 24 h, ivermectin paralysed J2 s. Counterintuitively, over the same time‐course ivermectin increased the rate of depletion of J2 lipid, suggesting that in ivermectin‐treated J2 s there is a disconnection between the energy requirements for motility and metabolic rate. This decrease in lipid stores would be predicted to negatively impact on J2 infective potential. These data suggest that the benefit of macrocyclic lactones as seed treatments may be underpinned by a multilevel effect involving both neuromuscular inhibition and acceleration of lipid metabolism. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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