The microbial nitrogen cycling potential is impacted by polyaromatic hydrocarbon pollution of marine sediments.
The microbial nitrogen cycling potential is impacted by polyaromatic hydrocarbon pollution of marine sediments.
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DOI：
10.3389/fmicb.2014.00108
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发表时间：
2014
影响因子：
5.2
通讯作者：
Gilbert JA
中科院分区：
文献类型：
--
作者：
Scott NM;Hess M;Bouskill NJ;Mason OU;Jansson JK;Gilbert JA
在碳氢化合物暴露期间，海洋微生物群落的组成和功能动态发生了变化，有利于能够利用这种丰富碳源的细菌。好氧表层沉积物中高水平碳氢化合物的初始暴露可以富集具有碳氢化合物降解能力的异养微生物的生长。因此，海洋沉积物表层内的氧势可能局部降低，造成厌氧区。我们假设，增加暴露于高浓度的碳氢化合物将与反硝化过程的增加和二氮的净积累呈正相关。通过比较6个宏基因组中与氮代谢和氮循环相关的基因的相对丰度来验证这一假设，其中6个宏基因组来自墨西哥湾深水地平线（DWH）漏油事件中受多环芳烃污染的沉积物，3个宏基因组来自圣巴巴拉海峡天然油渗漏相关的沉积物。分析了来自墨西哥湾未污染沉积物的另外8个宏基因组以进行比较。我们预测代谢物周转率的相对变化作为一个功能的差异微生物基因丰度，这表明预测的积累与反硝化过程相关的代谢物，包括厌氧氨氧化，在污染的样品相比，未污染的沉积物，这种变化的幅度正相关的碳氢化合物浓度和暴露时间。这些数据突出了碳氢化合物输入对海洋沉积物中氮循环过程的潜在影响，并为受影响生态系统中氮代谢的系统尺度模型提供了相关信息。
During hydrocarbon exposure, the composition and functional dynamics of marine microbial communities are altered, favoring bacteria that can utilize this rich carbon source. Initial exposure of high levels of hydrocarbons in aerobic surface sediments can enrich growth of heterotrophic microorganisms having hydrocarbon degradation capacity. As a result, there can be a localized reduction in oxygen potential within the surface layer of marine sediments causing anaerobic zones. We hypothesized that increasing exposure to elevated hydrocarbon concentrations would positively correlate with an increase in denitrification processes and the net accumulation of dinitrogen. This hypothesis was tested by comparing the relative abundance of genes associated with nitrogen metabolism and nitrogen cycling identified in 6 metagenomes from sediments contaminated by polyaromatic hydrocarbons from the Deepwater Horizon (DWH) oil spill in the Gulf of Mexico, and 3 metagenomes from sediments associated with natural oil seeps in the Santa Barbara Channel. An additional 8 metagenomes from uncontaminated sediments from the Gulf of Mexico were analyzed for comparison. We predicted relative changes in metabolite turnover as a function of the differential microbial gene abundances, which showed predicted accumulation of metabolites associated with denitrification processes, including anammox, in the contaminated samples compared to uncontaminated sediments, with the magnitude of this change being positively correlated to the hydrocarbon concentration and exposure duration. These data highlight the potential impact of hydrocarbon inputs on N cycling processes in marine sediments and provide information relevant for system scale models of nitrogen metabolism in affected ecosystems.
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影响因子：
14.9
作者：
Benson DA;Cavanaugh M;Clark K;Karsch-Mizrachi I;Ostell J;Pruitt KD;Sayers EW
通讯作者：
Sayers EW
影响因子：
4.5
作者：
Engstrom, Pia;Penton, C. Ryan;Devol, Allan H.
通讯作者：
Devol, Allan H.
影响因子：
4
作者：
Karl, D;Michaels, A;Stal, L
通讯作者：
Stal, L
影响因子：
56.9
作者：
Hazen, Terry C.;Dubinsky, Eric A.;Mason, Olivia U.
通讯作者：
Mason, Olivia U.
影响因子：
4.4
作者：
Bordenave, Sylvain;Goni-Urriza, Maria Soledad;Duran, Robert
通讯作者：
Duran, Robert