How yeast re-programmes its transcriptional profile in response to different nutrient impulses.
How yeast re-programmes its transcriptional profile in response to different nutrient impulses.
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DOI：
10.1186/1752-0509-5-148
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发表时间：
2011-09-25
影响因子：
--
通讯作者：
Oliver SG
中科院分区：
文献类型：
--
作者：
Dikicioglu D;Karabekmez E;Rash B;Pir P;Kirdar B;Oliver SG
微生物能够适应其物理化学或营养环境的变化，这对其生存至关重要。酵母，酿酒酵母，已经开发出一种机制，以快速有效的方式应对这种环境变化;这种反应可能需要广泛的基因活性重新编程。对S.通过跟踪酵母转录组学谱的短期和长期变化，研究了酿酒酵母对突然和瞬时去除碳或氮限制的响应。这项研究的时间跨度从几秒到几小时，揭示了代谢和遗传调控开关的层次结构，这些开关允许酵母适应以前限制营养素的脉冲并从中恢复。在转录组水平上，葡萄糖脉冲引起与糖酵解、羧酸代谢、氧化磷酸化、核酸和硫代谢有关的基因表达的显著变化。在铵限制培养中，铵脉冲导致参与氮代谢和离子转运的基因表达的显着变化。虽然这两种扰动引起的显着变化，在蛋白质合成的机器和过程中所涉及的基因的表达，转录组反应延迟，在铵脉冲的情况下，不那么复杂。通过两种不同的系统级，基于网络的方法的监管事件的分析提供了进一步的信息动态组织的酵母细胞作为一个响应营养变化。这项研究提供了重要的信息的时间组织的转录组组织和潜在的调控事件作为对碳和氮脉冲。它还揭示了对放松营养限制的反应进行长期动态分析的重要性，以了解细胞动态行为的分子基础。
A microorganism is able to adapt to changes in its physicochemical or nutritional environment and this is crucial for its survival. The yeast, Saccharomyces cerevisiae, has developed mechanisms to respond to such environmental changes in a rapid and effective manner; such responses may demand a widespread re-programming of gene activity. The dynamics of the re-organization of the cellular activities of S. cerevisiae in response to the sudden and transient removal of either carbon or nitrogen limitation has been studied by following both the short- and long-term changes in yeast's transcriptomic profiles. The study, which spans timescales from seconds to hours, has revealed the hierarchy of metabolic and genetic regulatory switches that allow yeast to adapt to, and recover from, a pulse of a previously limiting nutrient. At the transcriptome level, a glucose impulse evoked significant changes in the expression of genes concerned with glycolysis, carboxylic acid metabolism, oxidative phosphorylation, and nucleic acid and sulphur metabolism. In ammonium-limited cultures, an ammonium impulse resulted in the significant changes in the expression of genes involved in nitrogen metabolism and ion transport. Although both perturbations evoked significant changes in the expression of genes involved in the machinery and process of protein synthesis, the transcriptomic response was delayed and less complex in the case of an ammonium impulse. Analysis of the regulatory events by two different system-level, network-based approaches provided further information about dynamic organization of yeast cells as a response to a nutritional change. The study provided important information on the temporal organization of transcriptomic organization and underlying regulatory events as a response to both carbon and nitrogen impulse. It has also revealed the importance of a long-term dynamic analysis of the response to the relaxation of a nutritional limitation to understand the molecular basis of the cells' dynamic behaviour.
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DOI：
10.1073/pnas.011404098
发表时间：
2001-01-02
影响因子：
11.1
作者：
Li, C;Wong, WH
通讯作者：
Wong, WH
影响因子：
12.3
作者：
Geladé R;Van de Velde S;Van Dijck P;Thevelein JM
通讯作者：
Thevelein JM
影响因子：
4.5
作者：
Beilharz, Traude H.;Preiss, Thomas
通讯作者：
Preiss, Thomas
影响因子：
10.5
作者：
FORSBURG, SL;GUARENTE, L
通讯作者：
GUARENTE, L
影响因子：
3.3
作者：
Causton, HC;Ren, B;Young, RA
通讯作者：
Young, RA