Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons.
Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons.
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DOI：
10.1016/j.resmic.2013.06.005
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发表时间：
2013-10
影响因子：
2.6
通讯作者：
Monfort, Patrick
中科院分区：
文献类型：
--
作者：
Cantet, Franck;Hervio-Heath, Dominique;Caro, Audrey;Le Mennec, Cecile;Monteil, Caroline;Quemere, Catherine;Jolivet-Gougeon, Anne;Colwell, Rita R.;Monfort, Patrick
副溶血性弧菌、创伤弧菌和霍乱弧菌是人类的病原体。对这些弧菌知之甚少。在法国沿海的泻湖里本研究的目的是调查他们的发病率在水，贝类和沉积物的三个法国地中海沿岸泻湖使用最可能的数字聚合酶链反应（MPN-PCR）。夏季，三个泻湖水体、沉积物、贻贝和文蛤中的副溶血性弧菌总数分别为1 ~>1.1 × 103 MPN/l、0.09 ~ 1.1 × 103 MPN/ml、9 ~ 210 MPN/g和1.5 ~ 2.1MPN/g。在冬季，除贻贝外，所有样品都含有副溶血性弧菌，但浓度很低。致病性（tdh或trh 2阳性）副溶血性弧菌存在于从这些泻湖收集的水，沉积物和贝类样品。在夏季，水体、沉积物和贝类样品中的创伤弧菌数量分别为1 ~ 1.1 × 103 MPN/l、0.07 ~ 110 MPN/ml和0.04 ~ 15 MPN/g。冬季未检出创伤弧菌。夏季水体和沉积物中很少检出霍乱弧菌。总之，本研究的结果突出了三种人类致病性弧菌属的发现。存在于泻湖中，构成潜在的公共卫生危害。
Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 × 103 MPN/l, 0.09 to 1.1 × 103 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were present in water, sediment and shellfish samples collected from these lagoons. The number of V. vulnificus in water, sediment and shellfish samples ranged from 1 to 1.1 × 103 MPN/l, 0.07 to 110 MPN/ml and 0.04 to 15 MPN/g, respectively, during summer. V. vulnificus was not detected during winter. V. cholerae was rarely detected in water and sediment during summer. In summary, results of this study highlight the finding that the three human pathogenic Vibrio spp. are present in the lagoons and constitute a potential public health hazard.
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影响因子：
6.4
作者：
Daniels, NA;MacKinnon, L;Slutsker, L
通讯作者：
Slutsker, L
影响因子：
4
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影响因子：
4.4
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Garcia-Martin, Oscar
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作者：
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