Rift Valley Fever Virus Primes Immune Responses in Aedes aegypti Cells.
Rift Valley Fever Virus Primes Immune Responses in Aedes aegypti Cells.
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DOI：
10.3390/pathogens12040563
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发表时间：
2023-04-06
期刊：
影响因子：
--
通讯作者：
Dietrich I
中科院分区：
文献类型：
--
作者：
Laureti M;Lee RX;Bennett A;Wilson LA;Sy VE;Kohl A;Dietrich I
节肢动物传播病毒在全球范围内的不断出现加速了对这些病毒与其载体免疫系统相互作用的研究。只有有限的信息存在布尼亚病毒，如裂谷热病毒（RVFV），是如何通过蚊子的免疫或逃脱检测。RVFV是一种具有兽医和人类公共卫生和经济重要性的人畜共患静脉病毒（布尼亚病毒目;伪狂犬病毒科）。我们已经表明，蚊子感染RVFV触发RNA干扰途径的激活，这适度限制了病毒的复制。在这里，我们的目的是更好地了解RVFV和其他可能影响RVFV复制和传播的载体免疫信号通路之间的相互作用。为此，我们使用具有免疫活性的埃及伊蚊Aag 2细胞系作为模型。我们发现细菌诱导的免疫反应限制了RVFV的复制。然而，单独的病毒感染并不改变免疫效应物的基因表达水平。相反，它导致对随后细菌刺激的免疫反应显著增强。RVFV感染改变了几种蚊子免疫模式识别受体的基因表达水平，这可能有助于这种免疫启动。我们的研究结果表明，RVFV和蚊子免疫之间存在复杂的相互作用，可以在疾病预防策略中作为目标。
The ongoing global emergence of arthropod-borne (arbo) viruses has accelerated research into the interactions of these viruses with the immune systems of their vectors. Only limited information exists on how bunyaviruses, such as Rift Valley fever virus (RVFV), are sensed by mosquito immunity or escape detection. RVFV is a zoonotic phlebovirus (Bunyavirales; Phenuiviridae) of veterinary and human public health and economic importance. We have shown that the infection of mosquitoes with RVFV triggers the activation of RNA interference pathways, which moderately restrict viral replication. Here, we aimed to better understand the interactions between RVFV and other vector immune signaling pathways that might influence RVFV replication and transmission. For this, we used the immunocompetent Aedes aegypti Aag2 cell line as a model. We found that bacteria-induced immune responses restricted RVFV replication. However, virus infection alone did not alter the gene expression levels of immune effectors. Instead, it resulted in the marked enhancement of immune responses to subsequent bacterial stimulation. The gene expression levels of several mosquito immune pattern recognition receptors were altered by RVFV infection, which may contribute to this immune priming. Our findings imply that there is a complex interplay between RVFV and mosquito immunity that could be targeted in disease prevention strategies.
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影响因子：
10.5
作者：
Harrison, DA;McCoon, PE;Perrimon, N
通讯作者：
Perrimon, N
影响因子：
4.6
作者：
Brown, S;Hu, N;Hombría, JCG
通讯作者：
Hombría, JCG
白纹伊蚊中效应子半胱天冬酶 AaCASPS7 的鉴定
影响因子：
3.5
作者：
Feng, Lingyan;Liu, Hao;Liu, Qingzhen
通讯作者：
Liu, Qingzhen
DOI：
10.1073/pnas.1412984112
发表时间：
2015-01-13
影响因子：
11.1
作者：
Carissimo, Guillaume;Pondeville, Emilie;Vernick, Kenneth D.
通讯作者：
Vernick, Kenneth D.
载体和非载体细胞对正胞厅病毒的抗病毒RNAi反应。
影响因子：
3.8
作者：
Dietrich I;Shi X;McFarlane M;Watson M;Blomström AL;Skelton JK;Kohl A;Elliott RM;Schnettler E
通讯作者：
Schnettler E