Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy.
Differential expression of ileal Na(+)/H(+) exchanger isoforms after enterectomy.
复制标题
肠切除术后回肠Na( )/H( )交换亚型的差异表达。
DOI：
10.1006/jsre.1999.5720
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Warner,BW
中科院分区：
文献类型：
--
作者：
FalconeJr,RA;Shin,CE;Stern,LE;Wang,Z;Erwin,CR;Soleimani,M;Warner,BW
BackgroundNa+/H+exchangers (NHE) are transporters involved in the absorption of NaCl along the gastrointestinal tract. The aim of this study was to determine the expression pattern of the intestinal brush border NHE isoforms 2 and 3 following massive small bowel resection (SBR). Additionally, the effect of epidermal growth factor (EGF) and salivarectomy (removal of the primary source of EGF) on the expression pattern was studied.Materials and methodsICR mice underwent a proximal SBR or sham surgery and then received either orogastric saline or EGF (50 μg/kg/day). In separate experiments mice underwent salivarectomy followed by SBR or sham. Postoperatively the remaining ileum was isolated and levels of NHE-2 and NHE-3 mRNA and protein were resolved.ResultsFollowing SBR, the expression of both mRNA and protein for NHE-3 increased by ∼2.5-fold. Treatment with EGF enhanced NHE-3 mRNA in sham animals with further elevation following SBR. The expression of NHE-2 mRNA demonstrated minimal change while protein marginally increased (40%) following SBR. EGF did not affect the expression of NHE-2 mRNA. Salivarectomy did not influence NHE-2 protein expression and inhibited the increased NHE-3 protein expression following SBR.ConclusionsFollowing SBR, the expression pattern for brush border NHE isoforms is distinctive. Increased expression of NHE-3 secondary to SBR and/or EGF treatment with loss of this increase following salivarectomy implies a common mechanism to enhance enterocyte proliferation and luminal absorption of NaCl and water. These results suggest that NHE-3 is an important ileal exchanger following SBR.
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