Altered miRNA expression in sputum for diagnosis of non-small cell lung cancer.
Altered miRNA expression in sputum for diagnosis of non-small cell lung cancer.
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DOI：
10.1016/j.lungcan.2009.04.004
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发表时间：
2010-02
期刊：
影响因子：
5.3
通讯作者：
Jiang, Feng
中科院分区：
文献类型：
--
作者：
Xie, Ying;Todd, Nevins W.;Liu, Zhenqiu;Zhan, Min;Fang, HongBin;Peng, Hong;Alattar, Mohammed;Deepak, Janaki;Stass, Sanford A.;Jiang, Feng
生物体液中的分子遗传标记分析已被提出作为一种有用的癌症诊断工具。MicroRNAs （miRNAs）是一种小的调节性rna，在肺癌中经常出现失调，并且已经显示出作为其预后的基于组织的标记物的前景。本研究的目的是确定miRNA异常表达是否可以作为痰标本中非小细胞肺癌（NSCLC）诊断的标志物。实验设计：采用实时逆转录聚合酶链反应（RT-PCR）检测23例非小细胞肺癌患者和17例无癌患者痰液中成熟miRNA mir-21和mir-155的表达，并与对照miRNA U6B归一化。将这些数据与传统的痰细胞学诊断肺癌进行比较。所有内源性mirna都以非常稳定的形式存在于痰中，并且可以通过实时RT-PCR进行敏感和特异性检测。肿瘤患者痰标本中Mir-21的表达量（76.32±9.79）明显高于无癌患者（62.24±3.82）（p<0.0001）。此外，mir-21的过表达表现出高度判别性的接受者-操作者特征（ROC）曲线，其ROC曲线下面积为0.902±0.054，明显区分癌症患者和非癌症患者。检测mir-21表达对肺癌诊断的敏感性为69.66%，特异性为100.00%，而痰细胞学检测的敏感性为47.82%，特异性为100.00%。痰中miRNA表达变化的测量可能是一种有用的无创肺癌诊断方法。
Analysis of molecular genetic markers in biological fluids has been proposed as a useful tool for cancer diagnosis. MicroRNAs (miRNAs) are small regulatory RNAs that are frequently dysregulated in lung cancer and have shown promise as tissue-based markers for its prognostication. The aim of this study was to determine whether aberrant miRNA expression can be used as a marker in sputum specimen for the diagnosis of non-small cell lung cancer (NSCLC). Experimental Design: Expressions of mature miRNAs, mir-21 and mir-155, were examined by real-time reverse transcription polymerase chain reaction (RT-PCR) and normalized to that of control miRNA, U6B, in sputum of 23 patients with NSCLC and 17 cancer-free subjects. The data was compared with conventional sputum cytology for the diagnosis of lung cancer. All endogenous miRNAs were present in sputum in a remarkably stable form and sensitively and specifically detected by real-time RT-PCR. Mir-21 expression in the sputum specimens was significantly higher in cancer patients (76.32 ± 9.79) than cancer-free individuals (62.24±3.82) (p<0.0001). Furthermore, overexpression of mir-21 showed highly discriminative receiver-operator characteristic (ROC) curve profile, clearly distinguishing cancer patients from cancer-free subjects with areas under the ROC curve at 0.902 ± 0.054. Detection of mir-21 expression produced 69.66% sensitivity and 100.00% specificity in diagnosis of lung cancer, as compared with 47.82% sensitivity and 100.00% specificity by sputum cytology. The measurement of altered miRNA expression in sputum could be a useful noninvasive approach for the diagnosis of lung cancer.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
6.2
作者：
Qiu Q;Todd NW;Li R;Peng H;Liu Z;Yfantis HG;Katz RL;Stass SA;Jiang F
通讯作者：
Jiang F
DOI：
10.1073/pnas.0703942104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Gironella, Meritxell;Seux, Mylene;Dusetti, Nelson J.
通讯作者：
Dusetti, Nelson J.
DOI：
10.1073/pnas.0804549105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Mitchell, Patrick S.;Parkin, Rachael K.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh