Magnetic enrichment of bronchial epithelial cells from sputum for lung cancer diagnosis.
Magnetic enrichment of bronchial epithelial cells from sputum for lung cancer diagnosis.
复制标题
作者：
Qiu Q;Todd NW;Li R;Peng H;Liu Z;Yfantis HG;Katz RL;Stass SA;Jiang F
通过对脱落的气道上皮细胞进行细胞学和分子遗传学分析，痰液是一种易于获得的肺癌早期诊断材料。然而，痰的使用受到其细胞异质性的限制，其中包括约95%的巨噬细胞和中性粒细胞，仅约1%的支气管上皮细胞。我们建议获得浓缩和纯化的支气管上皮细胞，以提高痰样本中肺癌的早期检测。收集I期非小细胞肺癌患者、无癌吸烟者和健康非吸烟者的痰液。使用抗cd14和抗cd16抗体珠的磁辅助细胞分选（MACS）通过消耗痰中的巨噬细胞和中性粒细胞来富集支气管上皮细胞。荧光原位杂交（FISH）分析检测FHIT缺失和细胞学在富集标本中进行评估。从1.1%的起始群体中浓缩支气管上皮细胞至40%纯度，平均富集36倍，每个样品至少2.3 × 105个细胞。检测FHIT缺失对肺癌诊断的敏感性在富集的痰中为58%，而在未富集的痰中为42% （P = 0.02）。强化痰的细胞学检查敏感性为53%，而未强化痰的敏感性为39% （P = .03）。此外，与未处理的标本需要多达10个细胞离心片相比，分析只需要2个未富集的痰液的细胞离心片。支气管上皮细胞的富集可提高痰液对肺癌的诊断价值及遗传学和细胞学分析的效率。
Sputum is an easily accessible diagnostic material for lung cancer early detection by cytologic and molecular genetic analysis of exfoliated airway epithelial cells. However, the use of sputum is limited by its cellular heterogeneity, which includes >95% macrophages and neutrophils and only about 1% bronchial epithelial cells. We propose to obtain concentrated and purified bronchial epithelial cells to improve early detection of lung cancer in sputum samples. Sputum was collected from patients with stage I nonsmall-cell lung cancer, cancer-free smokers, and healthy nonsmokers. Magnetic-assisted cell sorting (MACS) with anti-CD14 and anti-CD16 antibody beads were used to enrich bronchial epithelial cells by depleting macrophages and neutrophils from sputum. Fluorescence in situ hybridization (FISH) analysis for detection of FHIT deletion and cytology were evaluated in the enriched specimens. The bronchial epithelial cells were concentrated to 40% purity from 1.1% of the starting population, yielding an average of 36-fold enrichment and at least 2.3 × 105 cells per sample. Detecting FHIT deletions for lung cancer diagnosis produced 58% sensitivity in the enriched sputum, whereas there was 42% sensitivity in the unenriched samples (P = .02). Cytologic examination of the enriched sputum resulted in 53% sensitivity, as compared with 39% sensitivity in unenriched sputum (P = .03). Furthermore, only 2 cytocentrifuge slides of the unenriched sputum were needed for the analyses, as compared with up to 10 cytocentrifuge slides required from the unprocessed specimens. The enrichment of bronchial epithelial cells could improve the diagnostic value of sputum and the efficiency of genetic and cytologic analysis of lung cancer.
登录
查看更多内容
影响因子：
3.7
作者：
Kraemer, PS;Sanchez, CA;Reid, BJ
通讯作者：
Reid, BJ
影响因子：
45.3
作者：
Endoh, H;Tomida, S;Mitsudomi, T
通讯作者：
Mitsudomi, T
DOI：
10.1164/ajrccm.161.2.9903097
发表时间：
2000-02-01
影响因子：
24.7
作者：
Belda, J;Leigh, R;Hargreave, FE
通讯作者：
Hargreave, FE
DOI：
10.1002/cyto.990110203
发表时间：
1990-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
MILTENYI, S;MULLER, W;RADBRUCH, A
通讯作者：
RADBRUCH, A
DOI：
10.1002/cyto.990070607
发表时间：
1986-11-01
期刊：
CYTOMETRY
影响因子：
--
作者：
ALBRIGHT, CD;FROST, JK;PRESSMAN, NJ
通讯作者：
PRESSMAN, NJ