The role of endophytic fungal individuals and communities in the decomposition of Pinus massoniana needle litter.
The role of endophytic fungal individuals and communities in the decomposition of Pinus massoniana needle litter.
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内生真菌个体和群落在马尾松针叶凋落物分解中的作用。
DOI：
10.1371/journal.pone.0105911
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Yuan Z;Chen L
内生真菌（FE）作为“先锋”分解者的作用最近得到了认识。然而，FE 对垫料损失的影响程度尚不清楚。 FE 介导的分解的遗传和酶学基础也很少得到解决。针对中国南方普遍存在的马尾松，评估了 FE 的种群和个体（重点是两种主要的 Lophodermium 类群）对针叶凋落物分解的影响。体内（微观）实验数据表明，FE 引发的垫料损失百分比与孵化时间线性相关，七个月后接近 60%。体外分解测试还证实，内生 Lophodermium 分离物在两个月内造成 14-22% 的质量损失。外酶（纤维素酶和漆酶，对木质纤维素降解很重要）的定性分析表明，几乎所有 Lophodermium 分离株均表现出中度或强阳性反应。此外，从 Lophodermium 分离株中扩增了 β-葡萄糖苷酶（糖苷水解酶家族 3，GH3）、漆酶和纤维二糖水解酶（GH7）基因的部分序列作为“功能标记”，以评估其木质纤维素分解活性的潜力。检测到三种不同的基因，表明其分解系统灵活而精致，富含有限元。我们的工作强调了这样一种可能性，即FE的腐生和内生可能是启动快速分解的先决条件，因此可能是Fes对凋落物分解的关键，至少在早期阶段是这样。还简要讨论了核心真菌分解者存在的潜在指标。
The role of fungal endophytes (FEs) as “pioneer” decomposers has recently been recognized; however, the extent to which FEs contribute to litter loss is less well understood. The genetic and enzymatic bases of FE-mediated decomposition have also rarely been addressed. The effects of populations and individuals (with an emphasis on two dominant Lophodermium taxa) of FEs on needle-litter decomposition were assessed for Pinus massoniana, a ubiquitous pine in southern China. Data from in vivo (microcosm) experiments indicated that the percentage of litter-mass loss triggered by FEs was linearly correlated with incubation time and approached 60% after seven months. In vitro decomposition tests also confirmed that endophytic Lophodermium isolates caused 14–22% mass loss within two months. Qualitative analysis of exoenzymes (cellulase and laccase, important for lignocellulose degradation) revealed that almost all of the Lophodermium isolates showed moderate or strong positive reactions. Furthermore, partial sequences of β-glucosidase (glycoside hydrolase family 3, GH3), laccase, and cellobiohydrolase (GH7) genes were amplified from Lophodermium isolates as “functional markers” to evaluate their potential for lignocellulolytic activity. Three different genes were detected, suggesting a flexible and delicate decomposition system rich in FEs. Our work highlights the possibility that the saprophytism and endophytism of FEs may be prerequisites to initiating rapid decomposition and thus may be key in Fes’ contribution to litter decomposition, at least in the early stage. Potential indicators of the presence of core fungal decomposers are also briefly discussed.
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影响因子：
2.9
作者：
Boberg, Johanna B.;Ihrmark, Katarina;Lindahl, Bjorn D.
通讯作者：
Lindahl, Bjorn D.
影响因子：
20.3
作者：
Koukol, Ondrej;Kolarik, Miroslav;Baldrian, Petr
通讯作者：
Baldrian, Petr
DOI：
10.1079/9781845935344.0092
发表时间：
2009-01-01
期刊：
APPLIED MYCOLOGY
影响因子：
--
作者：
Osono, Takashi;Hirose, Dai
通讯作者：
Hirose, Dai
影响因子：
4.4
作者：
Edwards, Ivan P.;Upchurch, Rima A.;Zak, Donald R.
通讯作者：
Zak, Donald R.
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD