Comparative Proteome Analysis Reveals Four Novel Polyhydroxybutyrate (PHB) Granule-Associated Proteins in Ralstonia eutropha H16
Comparative Proteome Analysis Reveals Four Novel Polyhydroxybutyrate (PHB) Granule-Associated Proteins in Ralstonia eutropha H16
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比较蛋白质组分析揭示了富养罗尔斯顿菌 H16 中的四种新型聚羟基丁酸酯 (PHB) 颗粒相关蛋白
DOI：
10.1128/aem.03791-14
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发表时间：
2015
影响因子：
4.4
通讯作者：
Jendrossek D
中科院分区：
文献类型：
--
作者：
Sznajder A;Pfeiffer D;Jendrossek D
鉴定存在于从真养罗尔斯通氏菌分离的聚羟基丁酸酯（PHB）颗粒级分中但不存在于可溶性、膜和膜相关级分中的蛋白质揭示了仅存在12种具有PHB特异性位置的多肽加上4种先前已知的具有多个位置的PHB相关蛋白。先前假设的PHB解聚酶同工酶（PhaZa 2至PhaZa 5，PhaZd 1和PhaZd 2）和两种已知的3-羟基丁酸酯低聚物水解酶（PhaZb和PhaZc）均不显著存在于分离的PHB颗粒中。四个多肽，发现尚未被确定在聚羟基丁酸颗粒。其中三种新蛋白质是推定的α/β-水解酶，其中两种（A0671和B1632）具有PHB合酶/解聚酶特征。第三种新的蛋白质（A0225）是马铃薯糖蛋白样磷脂酶，一种酶，尚未描述的任何聚羟基丁酸积累物种的聚羟基丁酸颗粒。第四种蛋白（A2001）没有功能，但其编码基因与phaB 2（乙酰乙酰辅酶A [CoA]还原酶）和phaC 2（PHB合成酶）形成操纵子，这与假定的PHB代谢功能一致。通过荧光显微镜观察构建的与增强型黄色荧光蛋白（eYFP）的融合蛋白，证实了这4个新蛋白在体内的定位。A0671和B1632的缺失对营养肉汤（NB）-葡萄糖酸盐细胞的静止生长期中的PHB动员能力有微小但可检测的影响，证实了两种蛋白质在PHB代谢中的功能参与。
Identification of proteins that were present in a polyhydroxybutyrate (PHB) granule fraction isolated from Ralstonia eutropha but absent in the soluble, membrane, and membrane-associated fractions revealed the presence of only 12 polypeptides with PHB-specific locations plus 4 previously known PHB-associated proteins with multiple locations. None of the previously postulated PHB depolymerase isoenzymes (PhaZa2 to PhaZa5, PhaZd1, and PhaZd2) and none of the two known 3-hydroxybutyrate oligomer hydrolases (PhaZb and PhaZc) were significantly present in isolated PHB granules. Four polypeptides were found that had not yet been identified in PHB granules. Three of the novel proteins are putative α/β-hydrolases, and two of those (A0671 and B1632) have a PHB synthase/depolymerase signature. The third novel protein (A0225) is a patatin-like phospholipase, a type of enzyme that has not been described for PHB granules of any PHB-accumulating species. No function has been ascribed to the fourth protein (A2001), but its encoding gene forms an operon withphaB2(acetoacetyl-coenzyme A [CoA] reductase) andphaC2(PHB synthase), and this is in line with a putative function in PHB metabolism. The localization of the four new proteins at the PHB granule surface was confirmedin vivoby fluorescence microscopy of constructed fusion proteins with enhanced yellow fluorescent protein (eYFP). Deletion of A0671 and B1632 had a minor but detectable effect on the PHB mobilization ability in the stationary growth phase of nutrient broth (NB)-gluconate cells, confirming the functional involvement of both proteins in PHB metabolism.
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影响因子：
3.2
作者：
Teruyuki Kobayashi;M. Shiraki;T. Abe;A. Sugiyama;Terumi Saito
通讯作者：
Teruyuki Kobayashi;M. Shiraki;T. Abe;A. Sugiyama;Terumi Saito
与 Herbaspirillum seropedicae SmR1 中的聚羟基丁酸酯颗粒相关的蛋白质的鉴定 - 老伙伴，新玩家
影响因子：
3.7
作者：
Evandro F. Tirapelle;M. Müller;M. Tadra;M. Kadowaki;M. Steffens;R. Monteiro;E. M. Souza;F. Pedrosa;L. S. Chubatsu
通讯作者：
L. S. Chubatsu
DOI：
--
发表时间：
1979
期刊：
European Journal of Applied Microbiology and Biotechnology
影响因子：
--
作者：
D. Vollbrecht;H. Schlegel;G. Stoschek;A. Janczikowski
通讯作者：
A. Janczikowski
来自真养产碱菌的三种不同蛋白质表现出 NAD 依赖性乙醛脱氢酶活性。
DOI：
--
发表时间：
1987
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
D. Jendrossek;A. Steinbüchel;H. Schlegel
通讯作者：
H. Schlegel
影响因子：
3.2
作者：
Yamada, Miwa;Yamashita, Koichi;Taguchi, Seiichi
通讯作者：
Taguchi, Seiichi