Electrochemiluminescence Immunosensor Based on Nanobody and Au/CaCO(3) Synthesized Using Waste Eggshells for Ultrasensitive Detection of Ochratoxin A.
Electrochemiluminescence Immunosensor Based on Nanobody and Au/CaCO(3) Synthesized Using Waste Eggshells for Ultrasensitive Detection of Ochratoxin A.
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基于纳米抗体和废蛋壳合成Au/CaCO3的电化学发光免疫传感器超灵敏检测赭曲霉毒素A
DOI：
10.1021/acsomega.1c05213
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发表时间：
2021-11-09
期刊：
影响因子：
4.1
通讯作者：
Yang DP
中科院分区：
文献类型：
--
作者：
Li L;Liu X;He S;Cao H;Su B;Huang T;Chen Q;Liu M;Yang DP
提出了一种基于Au/CaCO₃的新型超灵敏电化学发光（ECL）免疫分析法，用于检测咖啡中的赭曲霉毒素A（OTA）。以废弃蛋壳为模板合成的Au/CaCO₃纳米复合材料具有大的表面积和优异的电化学性能，被用于固定大量的Ru(bpy)₃²⁺以及偶联高亲和力的纳米抗体（通过噬菌体展示技术制备）。Au/CaCO₃纳米复合材料和纳米抗体技术的结合为OTA检测提供了一种超灵敏且高选择性的ECL免疫传感器，检测范围为10 pg/mL - 100 ng/mL，检测下限为5.7 pg/mL。此外，所制备的ECL免疫传感器表现出优异的性能和高稳定性。最后，将所提出的ECL传感器应用于分析咖啡样品中的OTA，证实了其理想的准确性和实际应用潜力。总体而言，这项工作展示了一种利用天然废弃物作为来源制备免疫传感器传感界面的新型纳米材料，以及一种检测食品中有毒OTA的方法。
A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO3 was proposed for detecting ochratoxin A (OTA) in coffee. Au/CaCO3 nanocomposites synthesized using waste eggshells as the template with a large surface area and excellent electrochemical properties were applied for immobilizing a large amount of Ru(bpy)32+ and conjugating a high-affinity nanobody (prepared by the phage display technique). Coupling of the Au/CaCO3 nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL–100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Finally, the proposed ECL sensor was applied to analyze OTA in coffee samples, confirming the desirable accuracy and practical applicability potential. Overall, this work presents a new nanomaterial for fabricating the sensing interface of immunosensors by harnessing natural waste as the source and a method for detecting toxic OTA in foods.
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影响因子：
6.7
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.saa.2020.118875
发表时间：
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影响因子：
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通讯作者：
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