Activation domain-mediated enhancement of activator binding to chromatin in mammalian cells.
Activation domain-mediated enhancement of activator binding to chromatin in mammalian cells.
复制标题
激活结构域介导的激活剂与哺乳动物细胞染色质结合的增强。
DOI：
10.1073/pnas.93.20.10820
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发表时间：
1996
影响因子：
11.1
通讯作者：
Kingston,RE
中科院分区：
文献类型：
--
作者：
Bunker,CA;Kingston,RE
DNA binding by transcriptional activators is typically an obligatory step in the activation of gene expression. Activator binding and subsequent steps in transcription are repressed by genomic chromatin. Studies in vitro have suggested that overcoming this repression is an important function of some activation domains. Here we provide quantitative in vivo evidence that the activation domain of GAL4-VP16 can increase the affinity of GAL4 for its binding site on genomic DNA in mammalian cells. Moreover, the VP16 activation domain has a much greater stimulatory effect on expression from a genomic reporter gene than on a transiently transfected reporter gene, where factor binding is more permissive. We found that not all activation domains showed a greater activation potential in a genomic context, suggesting that only some activation domains can function in vivo to alleviate the repressive effects of chromatin. These data demonstrate the importance of activation domains in relieving chromatin-mediated repression in vivo and suggest that one way they function is to increase binding of the activator itself.
c-Myc 和 Max 与核小体 DNA 的差异结合。
影响因子：
5.3
作者：
Wechsler,DS;Papoulas,O;Dang,CV;Kingston,RE
通讯作者：
Kingston,RE
人乳腺癌细胞中的小鼠乳腺肿瘤病毒染色质是组成性过敏的，并且在体内表现出不依赖类固醇激素的转录因子负载
影响因子：
5.3
作者：
J. Mymryk;D. Berard;G. Hager;T. Archer
通讯作者：
T. Archer
核小体组装蛋白 1 和核蓝蛋白刺激转录因子结合和组蛋白置换需要破坏组蛋白八聚体
影响因子：
5.3
作者：
P. P. Walter;Tom Owen;Jacques Côté;Jerry L. Workman
通讯作者：
Jerry L. Workman