Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.
Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.
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宿主蛋白与淋病奈瑟氏菌的广泛宿主范围噬菌体Ngoφ6的关联。
DOI：
10.1371/journal.pone.0240579
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Stein DC
中科院分区：
文献类型：
--
作者：
Piekarowicz A;Kłyż A;Adamczyk-Popławska M;Stein DC
所有淋病奈瑟菌菌株都含有多个拷贝的整合的丝状噬菌体基因组，其结构不确定。在这项研究中，我们试图表征的衣壳蛋白丝状N。淋病噬菌体NgoΦ6和在不同细菌中繁殖的噬菌粒。数据表明，纯化的噬菌体含有噬菌体编码的结构蛋白和细菌宿主蛋白;宿主蛋白始终与噬菌体颗粒共纯化。与噬菌体细丝相关的细菌宿主蛋白（如通过质谱法鉴定的）往往是宿主菌株的主要外膜组分之一，加上次要的额外宿主蛋白。我们能够与噬菌体细丝共纯化功能性β-内酰胺酶，一种噬菌粒编码的蛋白质。我们使用蛋白质建模和免疫学分析来鉴定噬菌体编码的主要结构蛋白。这些蛋白质的抗原特性取决于细菌的繁殖。抗N.淋病噬菌体NgoΦ6识别噬菌体编码的蛋白质，如果噬菌体在N.淋病或H.但如果在沙门氏菌或E.杆菌我们表明，从淋球菌和嗜血杆菌中分离的噬菌体细丝是糖基化的，这可能解释了所观察到的抗原多样性。总的来说，数据表明，虽然奈瑟氏球菌丝状噬菌体是类似于其他Inovirus的整体基因组组织，它们的能力，与宿主蛋白质密切相关，表明它们具有独特的表面特性，并通过一个迄今未知的分泌途径分泌。
All Neisseria gonorrhoeae strains contain multiple copies of integrated filamentous phage genomes with undefined structures. In this study, we sought to characterize the capsid proteins of filamentous N. gonorrhoeae bacteriophage NgoΦ6 and phagemids propagated in different bacteria. The data demonstrate that purified phage contain phage-encoded structural proteins and bacterial host proteins; host proteins consistently copurified with the phage particles. The bacterial host proteins associated with the phage filament (as identified by mass spectrometry) tended to be one of the predominant outer membrane components of the host strain, plus minor additional host proteins. We were able to copurify a functional ß-lactamase, a phagemid-encoded protein, with phage filaments. We used protein modeling and immunological analysis to identify the major phage encoded structural proteins. The antigenic properties of these proteins depended on the bacterium where the phages were propagated. Polyclonal antibodies against N. gonorrhoeae phage NgoΦ6 recognized phage-encoded proteins if the phage was propagated in N. gonorrhoeae or H. influenzae cells but not if it was propagated in Salmonella or E. coli. We show that the phage filaments isolated from gonococci and Haemophilus are glycosylated, and this may explain the antigenic diversity seen. Taken en toto, the data demonstrate that while the neisserial filamentous phage are similar to other Inovirus with respect to overall genomic organization, their ability to closely associate with host proteins suggests that they have unique surface properties and are secreted by a here-to-fore unknown secretory pathway.
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DOI：
10.1093/bioinformatics/btq662
发表时间：
2011-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Benkert P;Biasini M;Schwede T
通讯作者：
Schwede T
影响因子：
3.1
作者：
BLAKE, MS;BLAKE, CM;MANDRELL, RE
通讯作者：
MANDRELL, RE
影响因子：
4.9
作者：
OCALLAGHAN, CH;SHINGLER, AH;MORRIS, A
通讯作者：
MORRIS, A
影响因子：
3.5
作者：
INOUE, H;NOJIMA, H;OKAYAMA, H
通讯作者：
OKAYAMA, H
DOI：
10.1084/jem.20050112
发表时间：
2005-06-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bille E;Zahar JR;Perrin A;Morelle S;Kriz P;Jolley KA;Maiden MC;Dervin C;Nassif X;Tinsley CR
通讯作者：
Tinsley CR