Olfactory Rod Cells: A Rare Cell Type in the Larval Zebrafish Olfactory Epithelium With a Large Actin-Rich Apical Projection.

Olfactory Rod Cells: A Rare Cell Type in the Larval Zebrafish Olfactory Epithelium With a Large Actin-Rich Apical Projection.
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嗅杆细胞:斑马鱼幼鱼嗅上皮中一种罕见的细胞类型,具有大的富含肌动蛋白的顶端突起。

DOI:
10.3389/fphys.2021.626080
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发表时间:
2021
影响因子:
4
通讯作者:
Whitfield TT
Whitfield TT
中科院分区:
医学2区
文献类型:
--
作者:
Cheung KY;Jesuthasan SJ;Baxendale S;van Hateren NJ;Marzo M;Hill CJ;Whitfield TT

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我们报告存在一种罕见的细胞类型,嗅杆细胞,在发育中的斑马鱼嗅上皮。这些细胞每个都带有一个富含肌动蛋白的棒状突起,从上皮表面延伸5-10 μm。现场成像与无处不在的Lifeact-RFP标签表明,嗅杆可以振荡。嗅杆在嗅坑开放后几小时内出现,在幼虫阶段数量和大小增加,并且可以在没有嗅纤毛的情况下发育。嗅杆细胞在形态上不同于已知类别的嗅觉感觉神经元,但表达由神经元启动子驱动的报告基因。嗅杆细胞的亚群表达由sox 10启动子驱动的Lifeact-mRFPruby转基因。嵌合表达的转基因揭示,嗅杆细胞有圆形的细胞体位于顶部的嗅上皮细胞,并没有检测到轴突。我们在讨论中对这些细胞的可能功能进行了推测。
We report the presence of a rare cell type, the olfactory rod cell, in the developing zebrafish olfactory epithelium. These cells each bear a single actin-rich rod-like apical projection extending 5–10 μm from the epithelial surface. Live imaging with a ubiquitous Lifeact-RFP label indicates that the olfactory rods can oscillate. Olfactory rods arise within a few hours of the olfactory pit opening, increase in numbers and size during larval stages, and can develop in the absence of olfactory cilia. Olfactory rod cells differ in morphology from the known classes of olfactory sensory neuron, but express reporters driven by neuronal promoters. A sub-population of olfactory rod cells expresses a Lifeact-mRFPruby transgene driven by the sox10 promoter. Mosaic expression of this transgene reveals that olfactory rod cells have rounded cell bodies located apically in the olfactory epithelium and have no detectable axon. We offer speculation on the possible function of these cells in the Discussion.
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