Changes in the equine fecal microbiota associated with the use of systemic antimicrobial drugs.
Changes in the equine fecal microbiota associated with the use of systemic antimicrobial drugs.
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DOI：
10.1186/s12917-015-0335-7
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发表时间：
2015-02-03
影响因子：
2.6
通讯作者：
Weese JS
中科院分区：
文献类型：
--
作者：
Costa MC;Stämpfli HR;Arroyo LG;Allen-Vercoe E;Gomes RG;Weese JS
肠道是一个丰富而复杂的环境，其微生物群已被证明对宿主的健康和疾病具有重要作用。有几个因素可以导致正常肠道微生物群的破坏，包括抗菌治疗，这是马腹泻的重要原因。本研究旨在表征与常用抗菌药物给药相关的健康马粪便细菌种群的变化。24名成年患者被分配接受普鲁卡因青霉素肌内注射（IM）、头孢噻呋钠IM、甲氧苄啶磺胺嘧啶（TMS）口服或对照组。给药连续5天，并在给药前（第1天）、给药结束时（第5天）以及试验第14天和第30天采集粪便样本。使用Illumina MiSeq测序仪进行16 S rRNA基因的V4区的高通量测序。使用所有药物后，观察到人口结构和社区成员的显着变化。TMS引起粪便微生物群的最显着变化，即使在更高的分类水平，包括丰富度和多样性显着下降。这些变化主要是由于疣微菌属急剧减少，特别是“5个不确定的属”。抗菌药物给药引起的结构和成员的变化对每种药物都是特异性的，并且可以预测。治疗结束后25天，细菌谱与治疗前的模式更相似，表明从抗菌药物给药引起的变化中恢复，但差异仍然明显，特别是在社区成员方面。全身性抗菌药物的使用导致肠道微生物群的变化，对不同的抗菌药物有不同的特异性反应。这里测试的所有抗菌剂对微生物群都有一定的影响，但TMS显著降低了细菌物种丰富度和多样性，并对种群结构产生了最明显的影响，特别是针对疣微生物门的成员。本文的在线版本（doi：10.1186/s12917-015-0335-7）包含补充材料，可供授权用户使用。
The intestinal tract is a rich and complex environment and its microbiota has been shown to have an important role in health and disease in the host. Several factors can cause disruption of the normal intestinal microbiota, including antimicrobial therapy, which is an important cause of diarrhea in horses. This study aimed to characterize changes in the fecal bacterial populations of healthy horses associated with the administration of frequently used antimicrobial drugs. Twenty-four adult mares were assigned to receive procaine penicillin intramuscularly (IM), ceftiofur sodium IM, trimethoprim sulfadiazine (TMS) orally or to a control group. Treatment was given for 5 consecutive days and fecal samples were collected before drug administration (Day 1), at the end of treatment (Days 5), and on Days 14 and 30 of the trial. High throughput sequencing of the V4 region of the 16S rRNA gene was performed using an Illumina MiSeq sequencer. Significant changes of population structure and community membership were observed after the use of all drugs. TMS caused the most marked changes on fecal microbiota even at higher taxonomic levels including a significant decrease of richness and diversity. Those changes were mainly due to a drastic decrease of Verrucomicrobia, specifically the “5 genus incertae sedis”. Changes in structure and membership caused by antimicrobial administration were specific for each drug and may be predictable. Twenty-five days after the end of treatment, bacterial profiles were more similar to pre-treatment patterns indicating a recovery from changes caused by antimicrobial administration, but differences were still evident, especially regarding community membership. The use of systemic antimicrobials leads to changes in the intestinal microbiota, with different and specific responses to different antimicrobials. All antimicrobials tested here had some impact on the microbiota, but TMS significantly reduced bacterial species richness and diversity and had the greatest apparent impact on population structure, specifically targeting members of the Verrucomicrobia phylum. The online version of this article (doi:10.1186/s12917-015-0335-7) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.6
作者：
De La Cochetiere, M. F.;Durand, T.;Beaugerie, L.
通讯作者：
Beaugerie, L.
影响因子：
9.4
作者：
De La Cochetière, MF;Durand, T;Doré, J
通讯作者：
Doré, J
影响因子：
3.3
作者：
Faubladier, C.;Chaucheyras-Durand, F.;Julliand, V.
通讯作者：
Julliand, V.
DOI：
10.1016/j.ijantimicag.2013.07.008
发表时间：
2013-11-01
影响因子：
10.8
作者：
Ferran, Aude A.;Bibbal, Delphine;Bousquet-Melou, Alain
通讯作者：
Bousquet-Melou, Alain