Ribonucleoprotein organization of eukaryotic RNA. XV. Different nucleoprotein structures of globin messenger RNA sequences in nuclear and polyribosomal ribonucleoprotein particles.
Ribonucleoprotein organization of eukaryotic RNA. XV. Different nucleoprotein structures of globin messenger RNA sequences in nuclear and polyribosomal ribonucleoprotein particles.
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真核RNA的核糖核蛋白组织。
DOI：
10.1016/0022-2836(81)90377-6
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发表时间：
1981
影响因子：
5.6
通讯作者：
Munroe,SH
中科院分区：
文献类型：
--
作者：
Pederson,T;Munroe,SH
异质核RNA和多核糖体信使RNA都与细胞中的特定蛋白质组复合，分别形成称为hnRNP和mRNP的核糖核蛋白复合物。在目前的调查中，球蛋白mRNA序列的核蛋白结构的hnRNP和mRNP的探测与核酸酶消化的条件下，RNA-蛋白质重排被证明不会发生。用胰腺RNA酶对含有hnRNP和mRNP的Friend红白血病细胞RNP组分进行温和消化，导致珠蛋白mRNA同源序列优先耗尽，如通过存活RNA与珠蛋白互补DNA的杂交所测量的。对核酸酶的超敏反应代表了65%的球蛋白mRNA同源序列，其余35%保持相对的核酸酶抗性。通过EDTA释放去除多核糖体mRNP，然后在蔗糖梯度上重新分离hnRNP，消除了对核酸酶敏感的珠蛋白mRNA序列，有利于相对耐核酸酶的序列。这些结果表明，mRNA序列在多核糖体mRNP中比在核hnRNP颗粒中更核酸酶敏感。这意味着mRNA序列在从细胞核到细胞质的移动过程中的某个时刻经历了RNP结构的显著变化。
Heterogeneous nuclear RNA and polyribosomal messenger RNA are both complexed with specific sets of proteins in the cell, forming ribonucleoprotein complexes known as hnRNP and mRNP, respectively. In the present investigation, the nucleoprotein structures of globin mRNA sequences in hnRNP and mRNP were probed by digestion with nuclease, under conditions in which RNA-protein rearrangements were shown not to occur. Mild digestion with pancreatic RNAase of a Friend erythroleukemia cell RNP fraction containing both hnRNP and mRNP resulted in a preferential depletion of globin mRNA-homologous sequences, as measured by hybridization of the surviving RNA with globin complementary DNA. Hypersensitivity to nuclease typifies 65% of the globin mRNA-homologous sequences, with the other 35% remaining relatively nuclease-resistant. Removal of polyribosomal mRNP by release with EDTA, followed by re-isolation of hnRNP on a sucrose gradient eliminated the nuclease-hypersensitive class of globin mRNA sequences in favor of the relatively nuclease-resistant class. These results suggest that mRNA sequences are more nuclease-sensitive in polyribosomal mRNP than they are in nuclear hnRNP particles. The implication is that mRNA sequences undergo a significant change in RNP structure at some point during their movement from nucleus to cytoplasm.
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影响因子：
14.9
作者：
GREENBERG, JR
通讯作者：
GREENBERG, JR
RNA-RNA 剪接机制和基因表达控制模型。
DOI：
--
发表时间：
1979
期刊：
Genetical research
影响因子：
--
作者：
V. Murray;R. Holliday
通讯作者：
R. Holliday
影响因子：
11.4
作者：
BRANLANT, C;KROL, A;JACOB, M
通讯作者：
JACOB, M
克隆的兔和小鼠 β-珠蛋白基因的比较显示两个同源内含子对的强烈进化分歧
影响因子：
64.8
作者：
J. Berg;A. Ooyen;N. Mantei;A. Schamboeck;G. Grosveld;R. Flavell;C. Weissmann
通讯作者：
C. Weissmann
信使RNA生物合成和核结构。
影响因子：
--
作者：
T. Pederson
通讯作者：
T. Pederson