A medium-chain fatty acid as an alternative energy source in mouse preimplantation development.
A medium-chain fatty acid as an alternative energy source in mouse preimplantation development.
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DOI：
10.1038/srep00930
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发表时间：
2012
影响因子：
4.6
通讯作者：
Tomita, Masaru
中科院分区：
文献类型：
--
作者：
Yamada, Mitsutoshi;Takanashi, Kazumi;Hamatani, Toshio;Hirayama, Akiyoshi;Akutsu, Hidenori;Fukunaga, Tomoko;Ogawa, Seiji;Sugawara, Kana;Shinoda, Kosaku;Soga, Tomoyoshi;Umezawa, Akihiro;Kuji, Naoaki;Yoshimura, Yasunori;Tomita, Masaru
为了进一步优化植入前胚胎的培养，我们采用毛细管电泳飞行时间质谱（CE-TOFMS）对相关培养基进行了代谢组学分析。我们检测到28种代谢物：23种胚胎排泄代谢物，包括16种氨基酸和5种培养基衍生代谢物（例如，辛酸酯，中链脂肪酸（MCFA））。由于缺乏MCFA在哺乳动物植入前发育的信息，本研究探讨辛酸作为一种潜在的替代能源植入前胚胎培养。在缺乏脂肪酸、丙酮酸和葡萄糖的培养基中没有胚胎存活，但补充辛酸挽救了胚胎发育。免疫印迹结果显示，MCFA β-氧化的重要酶酰基辅酶A脱氢酶和羟酰基辅酶A脱氢酶在植入前胚胎中有明显表达。此外，CE-TOFMS追踪添加到培养基中的[1- 13 C8]辛酸盐通过线粒体中的β-氧化形成TCA循环的中间代谢物。这些结果是第一次证明，辛酸可以提供一个有效的替代能源在整个植入前的发展。
To further optimize the culturing of preimplantation embryos, we undertook metabolomic analysis of relevant culture media using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). We detected 28 metabolites: 23 embryo-excreted metabolites including 16 amino acids and 5 media-derived metabolites (e.g., octanoate, a medium-chain fatty acid (MCFA)). Due to the lack of information on MCFAs in mammalian preimplantation development, this study examined octanoate as a potential alternative energy source for preimplantation embryo cultures. No embryos survived in culture media lacking FAs, pyruvate, and glucose, but supplementation of octanoate rescued the embryonic development. Immunoblotting showed significant expression of acyl-CoA dehydrogenase and hydroxyacyl-CoA dehydrogenase, important enzymes for ß-oxidation of MCFAs, in preimplantation embryo. Furthermore, CE-TOFMS traced [1-13C8] octanoate added to the culture media into intermediate metabolites of the TCA cycle via ß-oxidation in mitochondria. These results are the first demonstration that octanoate could provide an efficient alternative energy source throughout preimplantation development.
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