Fluorophore-conjugated anti-CEA antibody for the intraoperative imaging of pancreatic and colorectal cancer.
Fluorophore-conjugated anti-CEA antibody for the intraoperative imaging of pancreatic and colorectal cancer.
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DOI：
10.1007/s11605-008-0581-0
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发表时间：
2008-11
影响因子：
3.2
通讯作者：
Bouvet, Michael
中科院分区：
文献类型：
--
作者：
Kaushal, Sharmeela;McElroy, Michele K.;Luiken, George A.;Talamini, Mark A.;Moossa, A. R.;Hoffman, Robert M.;Bouvet, Michael
关键词：
在美国，结直肠癌和胰腺癌分别是导致癌症相关死亡的第三和第四大常见原因。在这两种癌症中，在手术时完全检测原发和转移性病变对于最佳手术切除和适当的患者治疗至关重要。我们研究了使用荧光标记的抗癌胚胎抗原（CEA）单克隆抗体来帮助人类结直肠癌和胰腺癌裸鼠模型的肿瘤可视化。抗cea与绿色荧光基团结合。皮下、原位原发性和转移性胰腺和结直肠肿瘤在给予共轭抗cea后很容易用荧光成像显示。在全身抗体递送后30分钟可检测到荧光信号，并持续存在2周，暴露于标准手术室照明后，体内光漂白最小。肿瘤切除技术显示在荧光引导下切除标记肿瘤组织的能力有所提高。比较两种不同的荧光团揭示了剂量反应和体内光漂白的差异。这些结果表明，当CEA表达存在时，荧光团标记的抗CEA为增强结直肠癌和胰腺癌肿瘤的可视化提供了一种新的术中成像技术，并且荧光团的选择显著影响标记肿瘤的信号强度。
Colorectal and pancreatic cancers together comprise the third and fourth most common causes of cancer-related death in the United States. In both of these cancers, complete detection of primary and metastatic lesions at the time of surgery is critical to optimal surgical resection and appropriate patient treatment. We have investigated the use of fluorophore-labeled anti-carcinoembryonic antigen (CEA) monoclonal antibody to aid in cancer visualization in nude mouse models of human colorectal and pancreatic cancer. Anti-CEA was conjugated with a green fluorophore. Subcutaneous, orthotopic primary and metastatic human pancreatic and colorectal tumors were easily visualized with fluorescence imaging after administration of conjugated anti-CEA. The fluorescence signal was detectable 30 min after systemic antibody delivery and remained present for 2 weeks, with minimal in vivo photobleaching after exposure to standard operating room lighting. Tumor resection techniques revealed improved ability to resect labeled tumor tissue under fluorescence guidance. Comparison of two different fluorophores revealed differences in dose–response and photobleaching in vivo. These results indicate that fluorophore-labeled anti-CEA offers a novel intraoperative imaging technique for enhanced visualization of tumors in colorectal and pancreatic cancer when CEA expression is present, and that the choice of fluorophore significantly affects the signal intensity in the labeled tumor.
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影响因子：
6.2
作者：
Bria, Emilio;Milella, Michele;Giannarelli, Diana
通讯作者：
Giannarelli, Diana
影响因子：
9.6
作者：
ROBINSON, MHE;THOMAS, WM;MANGHAM, CM
通讯作者：
MANGHAM, CM
DOI：
10.1084/jem.122.3.467
发表时间：
1965-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gold P;Freedman SO
通讯作者：
Freedman SO
影响因子：
6.2
作者：
CONCANNON, JP;DALBOW, MH;LIEBLER, GA
通讯作者：
LIEBLER, GA
影响因子：
4.7
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
通讯作者：
Kobayashi, Hisataka