Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.
Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.
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DOI：
10.1016/j.psj.2023.103098
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发表时间：
2023-12
期刊：
影响因子：
4.4
通讯作者：
Song, Xiaokai
中科院分区：
文献类型：
--
作者：
Pu, Xianglin;Pan, Yangdong;Xiang, Quanjia;Lu, Mingmin;Xu, Lixin;Yan, Ruofeng;Li, Xiangrui;Song, Xiaokai
已有报道鸡球虫感染可抑制Th 1细胞因子IFN-γ的产生，从而逃避宿主免疫系统的清除。本研究旨在进一步研究巨型艾美耳球虫IFN-γ抑制分子（EmHPSP-2和EmHPSP-3）对鸡外周血单个核细胞（PBMC）及各T细胞亚群免疫功能的影响。首先，将分离的PBMC或分选的T细胞亚群用于与EmHPSP-2（rEmHPSP-2）和EmHPSP-3（rEmHPSP-3）的重组蛋白一起孵育。检测rEmHPSP-2和rEmHPSP-3对上述细胞增殖能力、一氧化氮（NO）释放和细胞因子mRNA水平的影响。CD 8+、CD 4 + CD 25 −、CD 4+和CD 4 + CD 25 + T细胞的分选纯度分别为93.01%、88.88%、87.04%和81.26%。rEmHPSP-2和rEmHPSP-3可显著抑制PBMC释放NO。rEmHPSP-2和rEmHPSP-3显著抑制PBMC和CD 4 + T细胞的增殖，而CD 8+、CD 4 + CD 25 −和CD 4 + CD 25 + T细胞则显著促进。2种蛋白均能显著下调PBMC中IFN-γ mRNA水平，上调PBMC中IL-10和TGF-β1的转录水平。IFN-γ和IL-2的转录水平在CD 8 + T细胞中被显著抑制。在CD 4 + CD 25 − T细胞中，rEmHPSP-2和rEmHPSP-3显著抑制IFN-γ转录水平，但促进IL-4。同时，rEmHPSP-2和rEmHPSP-3对含有CD 25+细胞的CD 4 + T细胞IFN-γ和IL-2转录水平的抑制作用较CD 25+细胞去除组更为明显。结果发现，IL-10、TGF-β1和细胞毒性T淋巴细胞相关抗原-4（CTLA-4）mRNA水平在蛋白质刺激鸡CD 4 + CD 25 + T细胞后显著上调。本研究不仅对阐明鸡球虫的免疫逃避机制具有重要意义，而且为鸡球虫病新型疫苗的研制提供了候选抗原分子。
It has been reported that infection of chicken coccidian could inhibit the production of Th1 cytokine IFN-γ, thereby evading clearance by the host immune system. The present study aimed to have a further investigation into the effects of Eimeria maxima IFN-γ inhibitory molecules (EmHPSP-2 and EmHPSP-3) on the immune function of chicken peripheral blood mononuclear cells (PBMC) and various T cell subsets. First, separated PBMC or sorted T cell subsets were used for incubation with recombinant proteins of EmHPSP-2 (rEmHPSP-2) and EmHPSP-3 (rEmHPSP-3). Subsequently, the effects of rEmHPSP-2 and rEmHPSP-3 on proliferative capacity, nitric oxide (NO) release and mRNA levels of cytokines of the above cells were detected. The sorting purity of CD8+, CD4+ CD25−, CD4+, and CD4+ CD25+ T cells was 93.01, 88.88, 87.04, and 81.26%, respectively. The NO release of PBMC was significantly inhibited by rEmHPSP-2 and rEmHPSP-3. The proliferation of PBMC and CD4+ T cells was significantly inhibited by rEmHPSP-2 and rEmHPSP-3, whereas CD8+, CD4+ CD25−, and CD4+ CD25+ T cells was significantly promoted by the 2 proteins. The 2 proteins significantly downregulated interferon-gamma (IFN-γ) mRNA level, upregulated the transcriptional levels of interleukin-10 (IL-10) and transforming growth factor-beta1 (TGF-β1) in PBMC. IFN-γ and IL-2 transcriptional levels were markedly inhibited in CD8+ T cells. IFN-γ transcriptional level was significantly inhibited, but IL-4 was promoted by rEmHPSP-2 and rEmHPSP-3 in CD4+ CD25− T cells. Meanwhile, the inhibitory effects of rEmHPSP-2 and rEmHPSP-3 on the transcriptional levels of IFN-γ and IL-2 were more obvious in CD4+ T cells containing CD25+ cells compared with the CD25+ cells depletion group. It was found that IL-10, TGF-β1, and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) mRNA levels were significantly upregulated upon stimulation of chicken CD4+ CD25+ T cells by proteins. This study is not only of great significance to clarify the immune evasion mechanism of chicken coccidia, but also provides candidate antigen molecules for development of a novel vaccine against chicken coccidiosis.
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影响因子：
2.1
作者：
Haritova, A. M.;Stanilova, S. A.
通讯作者：
Stanilova, S. A.
影响因子：
4.4
作者：
Maria Cardinal K;Kipper M;Andretta I;Machado Leal Ribeiro A
通讯作者：
Machado Leal Ribeiro A
DOI：
10.1016/j.ijpddr.2014.10.002
发表时间：
2014-12
影响因子：
4
作者：
Chapman, H. David;Jeffers, Thomas K.
通讯作者：
Jeffers, Thomas K.
影响因子：
7.3
作者：
da Fonseca-Martins AM;de Souza Lima-Gomes P;Antunes MM;de Moura RG;Covre LP;Calôba C;Rocha VG;Pereira RM;Menezes GB;Gomes DCO;Saraiva EM;de Matos Guedes HL
通讯作者：
de Matos Guedes HL
影响因子：
1.4
作者：
Allen, PC;Lillehoj, HS
通讯作者：
Lillehoj, HS