PEZO-1 and TRP-4 mechanosensors are involved in mating behavior in Caenorhabditis elegans.
PEZO-1 and TRP-4 mechanosensors are involved in mating behavior in Caenorhabditis elegans.
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DOI：
10.1093/pnasnexus/pgac213
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发表时间：
2022-11
期刊：
影响因子：
--
通讯作者：
Sternberg, Paul W.
中科院分区：
文献类型：
--
作者：
Brugman, Katherine I.;Susoy, Vladislav;Whittaker, Allyson J.;Palma, Wilber;Nava, Stephanie;Samuel, Aravinthan D. T.;Sternberg, Paul W.
秀丽隐杆线虫中的男性交配是一种复杂的行为，具有强大的机械感觉成分。秀丽隐杆线虫具有几种特征性的机械传感器蛋白，但很少有人证明有助于交配。在这里，我们研究了PEZO-1（压电通道PEZO-1）和TRP-4（一种机械转换TRPN通道）在男性交配行为中的作用。我们表明，PEZO-1在几个男性特异性神经元中表达，在交配中已知作用。我们表明，在其他神经元中，TRP-4在后羊皮后感觉神经元（PCA）感觉神经元中表达，该神经元在雄性和雌雄同体之间相对滑动，并抑制参与外阴检测的神经元。这两个基因的突变都损害了许多交配步骤，包括对雌雄同体，扫描，转弯和外阴检测的初始反应。在自由移动的突变雄性和雌雄同体之间交配过程中，我们进行了泛神经元成像。 PEZO-1和TRP-4突变体均显示出涉及外阴检测的感觉神经元的虚假激活。在TRP-4突变体中，这种虚假激活可能是由于PCA在扫描过程中抑制外阴探测神经元的抑制引起的。确实，我们表明，如果没有功能性TRP-4，PCA无法检测到男性和雌雄同体之间的相对滑动。细胞特异性TRP-4表达式恢复了PCA的机械感力函数。我们的结果证明了秀丽隐杆线虫交配行为中PEZO-1和TRP-4机械转换器的新作用。
Male mating in Caenorhabditis elegans is a complex behavior with a strong mechanosensory component. C. elegans has several characterized mechanotransducer proteins, but few have been shown to contribute to mating. Here, we investigated the roles of PEZO-1, a piezo channel, and TRP-4, a mechanotransducing TRPN channel, in male mating behavior. We show that pezo-1 is expressed in several male-specific neurons with known roles in mating. We show that, among other neurons, trp-4 is expressed in the Post-Cloacal sensilla neuron type A (PCA) sensory neuron, which monitors relative sliding between the male and the hermaphrodite and inhibits neurons involved in vulva detection. Mutations in both genes compromise many steps of mating, including initial response to the hermaphrodite, scanning, turning, and vulva detection. We performed pan-neuronal imaging during mating between freely moving mutant males and hermaphrodites. Both pezo-1 and trp-4 mutants showed spurious activation of the sensory neurons involved in vulva detection. In trp-4 mutants, this spurious activation might be caused by PCA failure to inhibit vulva-detecting neurons during scanning. Indeed, we show that without functional TRP-4, PCA fails to detect the relative sliding between the male and hermaphrodite. Cell-specific TRP-4 expression restores PCA's mechanosensory function. Our results demonstrate new roles for both PEZO-1 and TRP-4 mechanotransducers in C. elegans mating behavior.
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影响因子：
16.6
作者：
Coste, Bertrand;Murthy, Swetha E.;Mathur, Jayanti;Schmidt, Manuela;Mechioukhi, Yasmine;Delmas, Patrick;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem
影响因子：
2.6
作者：
Hughes, Kiley;Shah, Ashka;Vidal-Gadea, Andres G.
通讯作者：
Vidal-Gadea, Andres G.
DOI：
10.1093/jhered/esq003
发表时间：
2010-03-01
期刊：
The Journal of heredity
影响因子：
--
作者：
Anderson, Jennifer L;Morran, Levi T;Phillips, Patrick C
通讯作者：
Phillips, Patrick C
影响因子：
7.7
作者：
Bai, Xiaofei;Bouffard, Jeff;Golden, Andy
通讯作者：
Golden, Andy
影响因子：
3.4
作者：
Bae, Chilman;Gottlieb, Philip A.;Sachs, Frederick
通讯作者：
Sachs, Frederick