Changes in microtubule polarity orientation during the development of hippocampal neurons in culture.
Changes in microtubule polarity orientation during the development of hippocampal neurons in culture.
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DOI：
10.1083/jcb.109.6.3085
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
Banker GA
中科院分区：
文献类型：
--
作者：
Baas PW;Black MM;Banker GA
培养的海马神经元树突内的微管极性取向不一致。大约一半的微管具有朝向细胞体远端的正端，另一半具有朝向细胞体远端的负端;相比之下，轴突中的微管具有均匀的极性取向，全部具有朝向细胞体远端的正端（Baas，P.W.，J.S. Deitch，M. M. Black和G. A.银行家1988. Proc. Natl. Acad. Sci. USA. 85：8335-8339）。在这里，我们描述的发展变化，引起轴突和树突的独特的微管模式。培养的海马神经元最初延伸几个短突起，其中任何一个都可以明显地成为轴突（Dotti，C. G.，和G. A.银行家1987.自然[伦敦]。330：477-479）。轴突开始快速生长后几天，剩余的突起分化成树突（Dotti，C. G.，C. A. Sullivan和G. A.银行家1988.神经科学杂志8：1454-1468）。微管的极性取向在所有的初始过程中是一致的，与正末端的细胞体的远端，即使通过这些过程中的大多数将成为树突。这种均匀的微管极性取向在轴突生长的所有阶段都保持在轴突中。在其他过程中的微管的极性取向保持一致，直到它们开始生长并获得树突的形态特征。在此期间，在这些过程中，具有远离细胞体的负末端的微管首次出现。负末端远端微管的比例逐渐增加，直到7天的文化，大约相等数量的树突状微管在每个方向上的取向。因此，微管极性取向的区域差异的建立发生在神经元的初始极化之后，并且在时间上与树突的分化相关。
Microtubules in the dendrites of cultured hippocampal neurons are of nonuniform polarity orientation. About half of the microtubules have their plus ends oriented distal to the cell body, and the other half have their minus ends distal; in contrast, microtubules in the axon are of uniform polarity orientation, all having their plus ends distal (Baas, P.W., J.S. Deitch, M. M. Black, and G. A. Banker. 1988. Proc. Natl. Acad. Sci. USA. 85:8335-8339). Here we describe the developmental changes that give rise to the distinct microtubule patterns of axons and dendrites. Cultured hippocampal neurons initially extend several short processes, any one of which can apparently become the axon (Dotti, C. G., and G. A. Banker. 1987. Nature [Lond.]. 330:477-479). A few days after the axon has begun its rapid growth, the remaining processes differentiate into dendrites (Dotti, C. G., C. A. Sullivan, and G. A. Banker. 1988. J. Neurosci. 8:1454-1468). The polarity orientation of the microtubules in all of the initial processes is uniform, with plus ends distal to the cell body, even through most of these processes will become dendrites. This uniform microtubule polarity orientation is maintained in the axon at all stages of its growth. The polarity orientation of the microtubules in the other processes remains uniform until they begin to grow and acquire the morphological characteristics of dendrites. It is during this period that microtubules with minus ends distal to the cell body first appear in these processes. The proportion of minus end-distal microtubules gradually increases until, by 7 d in culture, about equal numbers of dendritic microtubules are oriented in each direction. Thus, the establishment of regional differences in microtubule polarity orientation occurs after the initial polarization of the neuron and is temporally correlated with the differentiation of the dendrites.
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DOI：
10.1083/jcb.109.4.1621
发表时间：
1989-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goslin K;Birgbauer E;Banker G;Solomon F
通讯作者：
Solomon F
影响因子：
64.8
作者：
DAVIS, L;BANKER, GA;STEWARD, O
通讯作者：
STEWARD, O
影响因子：
7.8
作者：
BAAS, PW;HEIDEMANN, SR
通讯作者：
HEIDEMANN, SR
DOI：
10.1073/pnas.76.1.514
发表时间：
1979-01-01
影响因子：
11.1
作者：
BOTTENSTEIN, JE;SATO, GH
通讯作者：
SATO, GH
DOI：
10.1083/jcb.84.1.141
发表时间：
1980-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bergen LG;Borisy GG
通讯作者：
Borisy GG