Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum.
Sociogenomics of self vs. non-self cooperation during development of Dictyostelium discoideum.
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DOI：
10.1186/1471-2164-15-616
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发表时间：
2014-07-21
期刊：
影响因子：
4.4
通讯作者：
Purugganan MD
中科院分区：
文献类型：
--
作者：
Li SI;Buttery NJ;Thompson CR;Purugganan MD
盘基网柄藻是一种社会进化的微生物模型，在嵌合体发育过程中具有区分自我和非自我的功能，并表现出基因型依赖的行为。然而，除了少量的细胞-细胞识别基因外，人们对这个物种自我/非自我识别的遗传基础知之甚少。基于D.由于discoideum细胞与非克隆配偶相互作用，我们在克隆与嵌合发育期间在该物种中进行了转录组学分析研究。D.使用定制的微阵列在五个不同的发育阶段比较克隆中的盘状突细胞与不同的嵌合体。嵌合体对与社会相互作用相关的全球转录模式的影响进行了观察。我们发现1,759个基因在嵌合体和克隆体之间存在显著差异，1,144个基因与显著的菌株差异相关，6,586个基因随时间的推移受到发育调控。主成分分析显示嵌合体相关因子的转录水平存在少量的变异（嵌合体：0.18%，嵌合体×时间点：0.03%）。有162个基因在嵌合体发育过程中受到特异性调控，在嵌合体与克隆体发育过程中存在持续的小差异。嵌合体相关差异基因中约60%的差异表达发生在4 h聚集期，即D.从单细胞到多细胞阶段。一个相对较小比例的基因表达的整体变化的解释嵌合和克隆发展之间的差异。与嵌合体相关的基因表达中相对较小的修饰与在不同的D.不同遗传背景的细胞将不加区别地共聚集并共同发育成子实体。嵌合体的发育可能涉及通过对发育遗传网络的小的修改来重新编程转录组，这也可能表明对社会相互作用的反应涉及许多具有单独小的转录效应的基因。本文的在线版本（doi：10.1186/1471-2164-15-616）包含补充材料，可供授权用户使用。
Dictyostelium discoideum, a microbial model for social evolution, is known to distinguish self from non-self and show genotype-dependent behavior during chimeric development. Aside from a small number of cell-cell recognition genes, however, little is known about the genetic basis of self/non-self recognition in this species. Based on the key hypothesis that there should be differential expression of genes if D. discoideum cells were interacting with non-clone mates, we performed transcriptomic profiling study in this species during clonal vs. chimeric development. The transcriptomic profiles of D. discoideum cells in clones vs. different chimeras were compared at five different developmental stages using a customized microarray. Effects of chimerism on global transcriptional patterns associated with social interactions were observed. We find 1,759 genes significantly different between chimera and clone, 1,144 genes associated significant strain differences, and 6,586 genes developmentally regulated over time. Principal component analysis showed a small amount of the transcriptional variance to chimerism-related factors (Chimerism: 0.18%, Chimerism × Timepoint: 0.03%). There are 162 genes specifically regulated under chimeric development, with continuous small differences between chimera vs. clone over development. Almost 60% of chimera-associated differential genes were differentially expressed at the 4 h aggregate stage, which corresponds to the initial transition of D. discoideum from solitary life to a multicellular phase. A relatively small proportion of over-all variation in gene expression is explained by differences between chimeric and clonal development. The relatively small modifications in gene expression associated with chimerism is compatible with the high level of cooperation observed among different strains of D. discoideum; cells of distinct genetic backgrounds will co-aggregate indiscriminately and co-develop into fruiting bodies. Chimeric development may involve re-programming of the transcriptome through small modifications of the developmental genetic network, which may also indicate that response to social interaction involves many genes with individually small transcriptional effect. The online version of this article (doi:10.1186/1471-2164-15-616) contains supplementary material, which is available to authorized users.
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影响因子：
4.5
作者：
Dumitriu A;Latourelle JC;Hadzi TC;Pankratz N;Garza D;Miller JP;Vance JM;Foroud T;Beach TG;Myers RH
通讯作者：
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