Inhibition of DNA replication by fish oil-treated cytoplasm is counteracted by fish oil-treated nuclear extract.
Inhibition of DNA replication by fish oil-treated cytoplasm is counteracted by fish oil-treated nuclear extract.
复制标题
鱼油处理的细胞质对 DNA 复制的抑制被鱼油处理的核提取物抵消。
DOI：
10.1152/ajpcell.00121.2002
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Istfan,NawfalW
中科院分区：
文献类型：
--
作者：
Rex,Sybille;Kukuruzinska,MariaA;Istfan,NawfalW
We have recently noted that cells treated with fish oil and n–3-fatty acids show slower DNA replication rates than cells treated with a control emulsion or corn oil only. However, it is not clearly understood how such an effect is induced. Fish oil and its metabolites are known to have several modulating effects on signal transduction pathways. Alternatively, they may influence DNA replication by interacting directly with nuclear components. To investigate this problem in greater detail, we have studied the kinetics of DNA synthesis in a cell-free system derived from HeLa cells. Nuclei and cytosolic extract were isolated from cells synchronized in early S phase after treatment with control emulsion, corn oil, or fish oil, respectively. The nuclei were reconstituted with cytosolic extract and a reaction mixture containing bromodeoxyuridine (BrdU) triphosphate to label newly synthesized DNA. The rate of DNA synthesis was measured by bivariate DNA/BrdU analysis and flow cytometry. We show that fish oil-treated cytosol inhibits the elongation of newly synthesized DNA by ∼80% in control nuclei. However, nuclei treated with fish oil escape this inhibitory effect. We also show that addition of nuclear extract from fish oil-treated cells reverses the inhibitory effect seen in the reconstitution system of control nuclei and fish oil-treated cytosol. These results indicate that polyunsaturated fatty acids can modulate DNA synthesis through cytosolic as well as soluble nuclear factors.
登录
查看更多内容
影响因子：
120.7
作者：
Holmes, MD;Hunter, DJ;Hankinson, SE
通讯作者：
Hankinson, SE
根据流式细胞术数据计算潜在倍增时间的改进方法。
DOI：
10.1002/cyto.990110214
发表时间：
1990
期刊：
Cytometry
影响因子：
--
作者：
White,RA;Terry,NH;Meistrich,ML;Calkins,DP
通讯作者：
Calkins,DP
DOI：
--
发表时间：
2000
期刊：
The Journal of Nutrition, Health & Aging
影响因子：
--
作者：
C. Gogos;A. Skoutelis;F. Kalfarentzos
通讯作者：
F. Kalfarentzos
病毒性肝炎标志物与慢性肝炎病理形态学和临床相关的研究。
DOI：
--
发表时间：
1995
期刊：
Romanian Journal of Virology
影响因子：
--
作者：
D. Brehar;Á. Vajda;D. Négréa;R. Străin;N. Tudose;I. Sporea;R. Moldovan;C. Chidiosan;O. Chirileanu;D. Gaiţă
通讯作者：
D. Gaiţă
膳食α-亚麻酸对两种小鼠乳腺癌生长、转移、脂肪酸谱和前列腺素产生的影响。
DOI：
--
发表时间：
1990
期刊：
Journal of NutriLife
影响因子：
--
作者：
K. Fritsche;P. V. Johnston
通讯作者：
P. V. Johnston