Bacterial antagonists of fungal pathogens also control root-knot nematodes by induced systemic resistance of tomato plants.
Bacterial antagonists of fungal pathogens also control root-knot nematodes by induced systemic resistance of tomato plants.
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DOI：
10.1371/journal.pone.0090402
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hallmann J
中科院分区：
文献类型：
--
作者：
Adam M;Heuer H;Hallmann J
在温室条件下研究了真菌病原体的细菌拮抗剂控制根结线虫南方根结线虫的潜力。与未处理的对照相比，用几种菌株处理番茄种子显着减少了虫瘿和卵块的数量。表现最好的枯草芽孢杆菌分离株 Sb4-23、Mc5-Re2 和 Mc2-Re2，进一步研究了它们在细菌代谢物或驱虫剂的直接作用以及植物介导作用方面的作用模式。与不使用培养物上清液的对照相比，用培养物上清液浇透土壤可显着减少番茄上南方根结线虫产生的卵块数量高达 62%。在相连的双盆装置中显示了拮抗剂对幼虫的排斥作用，其中大多数幼虫在没有接种拮抗剂的一侧穿透根部。所有测试的生物防治菌株均诱导番茄对南方根结线虫的系统抗性，正如在分根系统中所揭示的那样，其中细菌和线虫接种在同一植物的空间分离的根部。这使卵块的产量减少了高达 51%，而与单独诱导系统抗性相比，在同一盆中接种细菌和线虫对南方根结线虫的抑制仅具有较小的附加效应。因此，植物介导的作用是拮抗的主要原因，而不是直接机制。总之，已知具有对抗真菌病原体的拮抗潜力的细菌也能抑制南方根结线虫。这种“多用途”细菌可能为控制策略提供新的选择，特别是对于导致协同产量损失的线虫-真菌疾病复合体。
The potential of bacterial antagonists of fungal pathogens to control the root-knot nematode Meloidogyne incognita was investigated under greenhouse conditions. Treatment of tomato seeds with several strains significantly reduced the numbers of galls and egg masses compared with the untreated control. Best performed Bacillus subtilis isolates Sb4-23, Mc5-Re2, and Mc2-Re2, which were further studied for their mode of action with regard to direct effects by bacterial metabolites or repellents, and plant mediated effects. Drenching of soil with culture supernatants significantly reduced the number of egg masses produced by M. incognita on tomato by up to 62% compared to the control without culture supernatant. Repellence of juveniles by the antagonists was shown in a linked twin-pot set-up, where a majority of juveniles penetrated roots on the side without inoculated antagonists. All tested biocontrol strains induced systemic resistance against M. incognita in tomato, as revealed in a split-root system where the bacteria and the nematodes were inoculated at spatially separated roots of the same plant. This reduced the production of egg masses by up to 51%, while inoculation of bacteria and nematodes in the same pot had only a minor additive effect on suppression of M. incognita compared to induced systemic resistance alone. Therefore, the plant mediated effect was the major reason for antagonism rather than direct mechanisms. In conclusion, the bacteria known for their antagonistic potential against fungal pathogens also suppressed M. incognita. Such “multi-purpose” bacteria might provide new options for control strategies, especially with respect to nematode-fungus disease complexes that cause synergistic yield losses.
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影响因子：
1.8
作者：
Aballay, Erwin;Ordenes, Paula;Persson, Paula
通讯作者：
Persson, Paula
影响因子：
4.2
作者：
Adesina, Modupe F.;Grosch, Rita;Smalla, Kornelia
通讯作者：
Smalla, Kornelia
影响因子：
2.1
作者：
Koeberl, Martina;Ramadan, Elshahat M.;Berg, Gabriele
通讯作者：
Berg, Gabriele
影响因子：
4.1
作者：
Pengnoo, A;Wiwattanapattapee, R;Kanjanamaneesathian, M
通讯作者：
Kanjanamaneesathian, M
影响因子：
3.2
作者：
Martinuz, A.;Schouten, A.;Sikora, R. A.
通讯作者：
Sikora, R. A.