Excreted secreted products from the parasitic nematode Steinernema carpocapsae manipulate the Drosophila melanogaster immune response.
Excreted secreted products from the parasitic nematode Steinernema carpocapsae manipulate the Drosophila melanogaster immune response.
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DOI：
10.1038/s41598-022-18722-7
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发表时间：
2022-08-20
影响因子：
4.6
通讯作者：
Eleftherianos, Ioannis
中科院分区：
文献类型：
--
作者：
Jones, Kirah;Tafesh-Edwards, Ghada;Kenney, Eric;Toubarro, Duarte;Simoes, Nelson;Eleftherianos, Ioannis
卡波囊斯氏线虫是一种昆虫病原性线虫（EPN），可迅速感染并杀死广泛的昆虫宿主，并在感染的初始阶段与宿主免疫抑制有关。S. Carpocapsae感染以前被认为是其共生细菌;然而，很明显，线虫能够通过其排泄/分泌产物（ESP）独立地有效杀死其宿主。在这里，我们研究了成年黑腹果蝇免疫系统是如何调节响应S。carpocapsae ESP，以试图确定分离的化合物的个体致病贡献。我们发现S. carpocapsae ESPs降低了D.它们诱导某些抗菌肽编码基因的表达，并且它们引起雄性果蝇中酚氧化酶活性的显著降低和黑化反应的延迟。我们还报告S. carpocapsae ESP影响男性和女性个体的血细胞数量。我们的研究结果表明EPN ESP的操纵作用，并揭示性别特异性差异的主机对线虫感染因子的反应。这些发现是有益的，因为它们促进了我们对线虫致病性的分子基础和影响线虫-宿主相互作用的寄生虫组分的理解。
Steinernema carpocapsae is an entomopathogenic nematode (EPN) that rapidly infects and kills a wide range of insect hosts and has been linked to host immunosuppression during the initial stages of infection. The lethal nature of S. carpocapsae infections has previously been credited to its symbiotic bacteria; however, it has become evident that the nematodes are able to effectively kill their hosts independently through their excretion/secretion products (ESPs). Here we examined how the adult Drosophila melanogaster immune system is modulated in response to S. carpocapsae ESPs in an attempt to ascertain individual pathogenic contributions of the isolated compound. We found that the S. carpocapsae ESPs decrease the survival of D. melanogaster adult flies, they induce the expression of certain antimicrobial peptide-encoding genes, and they cause significant reduction in phenoloxidase enzyme activity and delay in the melanization response in males flies. We also report that S. carpocapsae ESPs affect hemocyte numbers in both male and female individuals. Our results indicate the manipulative role of EPN ESPs and reveal sex-specific differences in the host response against nematode infection factors. These findings are beneficial as they promote our understanding of the molecular basis of nematode pathogenicity and the parasite components that influence nematode-host interactions.
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影响因子：
6.7
作者：
Binggeli O;Neyen C;Poidevin M;Lemaitre B
通讯作者：
Lemaitre B
影响因子：
3.6
作者：
Cooper, Dustin;Wuebbolt, Caitlin;Eleftherianos, Ioannis
通讯作者：
Eleftherianos, Ioannis
影响因子：
2.9
作者：
Lu, Yuzhen;Su, Fanghua;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang
影响因子：
1.3
作者：
Lara-Reyes, Nancy;Guillermo Jimenez-Cortes, J.;Contreras-Garduno, Jorge
通讯作者：
Contreras-Garduno, Jorge
影响因子：
5.3
作者：
Hillyer, Julian F.;Strand, Michael R.
通讯作者：
Strand, Michael R.