Thrombospondin-1 inhibits ossification of tissue engineered cartilage constructed by ADSCs.
Thrombospondin-1 inhibits ossification of tissue engineered cartilage constructed by ADSCs.
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Thrombospondin-1 抑制 ADSC 构建的组织工程软骨的骨化。
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lanjun Nie
中科院分区：
文献类型：
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作者：
Aiguo Xie;Jixin Xue;Gan Shen;Lanjun Nie
软骨组织工程为软骨缺损的治疗提供了一种新的方法，而脂肪干细胞因其自身的特点，有望成为软骨组织工程的理想种子细胞。然而，组织工程软骨体内植入后的骨化仍然是一个挑战。凝血酶敏感蛋白-1具有抑制血管生成的作用，可能在抑制脂肪干细胞构建的组织工程软骨的成骨过程中发挥重要作用。因此，本研究对凝血酶敏感蛋白-1抑制组织工程软骨成骨的作用进行了研究。将携带凝血酶敏感蛋白-1基因的慢病毒载体导入脂肪干细胞，并将其用于实验。用定量逆转录聚合酶链式反应和免疫印迹法检测血栓反应蛋白-1的表达，通过血管生成实验分析血栓反应蛋白-1的过表达对血管生成的影响。用生物力学和分子生物学方法评价组织工程软骨的质量和骨化程度。结果表明，血栓反应蛋白-1感染的细胞在mRNA和蛋白水平均有较高的表达，抑制内皮细胞的管状形成，提示其具有抗血管生成作用。体外基因表达分析表明，凝血酶敏感蛋白-1显著抑制脂肪干细胞的成骨分化，体内实验结果表明，凝血酶敏感蛋白-1显著抑制成骨基因的表达。与对照组相比，体内转导血栓反应蛋白-1的脂肪干细胞构建的组织工程软骨具有较高的GAG含量和较低的压缩弹性系数，表明其成骨水平较低。综上所述，目前的研究表明，抗血管生成因子血栓反应蛋白-1在体外抑制脂肪干细胞的成骨分化，在体内抑制由脂肪干细胞构建的组织工程软骨的成骨。
Cartilage tissue engineering provides a new method in the treatment of cartilage defects, and adipose derived stem cells seem to be an ideal seed cell in cartilage tissue engineering because of its characteristics. However, ossification after in vivo implantation of tissue engineered cartilage remains a challenge. Thrombospondin-1 which has been reported to have an inhibitory effect on angiogenesis, may play an important role in inhibiting the ossification of tissue engineered cartilage constructed by adipose derived stem cells. Therefore, the effect of thrombospondin-1 in inhibiting the ossification of tissue engineered cartilage was evaluated in this study. Lentivirus vectors carrying thrombospondin-1 cDNA were transfected into adipose derived stem cells, and the transfected cells were used in the experiments. The expression of thrombospondin-1 was evaluated by quantitative reverse transcriptase-polymerase chain reaction and western blot, and the effects of thrombospondin-1 over-expression on angiogenesis were analyzed by angiogenesis assays. The quality of tissue engineered cartilage and the degree of ossification were assessed by biomechanical and molecular biology methods. The results showed that thrombospondin-1 infected cells have a high expression of thrombospondin-1 in mRNA and protein level, which inhibited the tube formation of endothelial cells, indicating the anti-angiogenic effects. Gene expression analyses in vitro showed that thrombospondin-1 inhibits the osteogenic differentiation of adipose derived stem cells significantly, and the results of in vivo study revealed that thrombospondin-1 significantly inhibits the expression of osteogenic genes. Compared to that in the control group, tissue engineered cartilage constructed by thrombospondin-1 transfected adipose derived stem cells in vivo showed a higher GAG content and lower compressive modulus, which indicating lower level of ossification. In conclusion, the current study indicated that the anti-angiogenic factor thrombospondin-1 suppresses the osteogenic differentiation of adipose derived stem cells in vitro, and inhibits ossification of tissue engineered cartilage constructed by adipose derived stem cells in vivo.
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影响因子：
3.2
作者：
A. Carbone;M. Valente;L. Annacontini;S. Castellani;S. Di Gioia;D. Parisi;M. Rucci;G. Belgiovine;C. Colombo;A. Di Benedetto;G. Mori;L. Lo Muzio;A. Maiorella;A. Portincasa;M. Conese
通讯作者：
A. Carbone;M. Valente;L. Annacontini;S. Castellani;S. Di Gioia;D. Parisi;M. Rucci;G. Belgiovine;C. Colombo;A. Di Benedetto;G. Mori;L. Lo Muzio;A. Maiorella;A. Portincasa;M. Conese
影响因子：
--
作者：
Palmisano NJ;Meléndez A
通讯作者：
Meléndez A
影响因子：
--
作者：
Lunli Gong;Xiao Zhou;Yaohao Wu;Yun Zhang;Chen Wang;Heng Zhou;Fangfang Guo;Lei Cui
通讯作者：
Lunli Gong;Xiao Zhou;Yaohao Wu;Yun Zhang;Chen Wang;Heng Zhou;Fangfang Guo;Lei Cui
影响因子：
1.9
作者：
Zhenghua Hong;Hai‐xiao Chen;Huaxing Hong;Lie Lin;Zhangfu Wang
通讯作者：
Zhenghua Hong;Hai‐xiao Chen;Huaxing Hong;Lie Lin;Zhangfu Wang
DOI：
10.1038/bonekey.2013.122
发表时间：
2013-08-07
期刊：
BoneKEy reports
影响因子：
--
作者：
Cohen-Zinder, Miri;Zinder-Cohen, Miri;Onn, Itay
通讯作者：
Onn, Itay