Mechanism of gram-positive shock: identification of peptidoglycan and lipoteichoic acid moieties essential in the induction of nitric oxide synthase, shock, and multiple organ failure.
Mechanism of gram-positive shock: identification of peptidoglycan and lipoteichoic acid moieties essential in the induction of nitric oxide synthase, shock, and multiple organ failure.
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DOI：
10.1084/jem.188.2.305
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发表时间：
1998-07-20
期刊：
影响因子：
--
通讯作者：
Thiemermann C
中科院分区：
文献类型：
--
作者：
Kengatharan KM;De Kimpe S;Robson C;Foster SJ;Thiemermann C
由革兰氏阳性菌引起的感染性休克的发病率在过去几年中显著上升。革兰氏阳性菌（不含内毒素）是如何引起休克和多器官衰竭的，目前还很不清楚。我们最近发现，致病性革兰氏阳性细菌金黄色葡萄球菌，脂磷壁酸（LTA）和肽聚糖（PepG）的两个细胞壁片段，协同作用，导致诱导一氧化氮（NO）的形成，休克和器官损伤的大鼠。我们在此报道PepG的特异性片段，N-乙酰葡糖胺-β-[1→ 4]-N-乙酰胞壁酰-L-丙氨酸-d-异谷氨酰胺，是PepG聚合物内负责与LTA（或细胞因子干扰素γ）协同作用以诱导小鼠巨噬细胞系J774.2中NO形成的部分。然而，该部分也存在于非致病性细菌枯草芽孢杆菌的PepG中。我们后来发现S.金黄色葡萄球菌LTA与来自任一种细菌的PepG协同作用，导致大鼠中NO形成增强、休克和器官损伤，而来自B.枯草芽孢杆菌不与任一细菌的PepG协同。因此，我们认为LTA的结构决定了特定细菌引起休克和多器官衰竭（致病性）的能力，而PepG则起着放大LTA诱导的任何反应的作用。
The incidence of septic shock caused by gram-positive bacteria has risen markedly in the last few years. It is largely unclear how gram-positive bacteria (which do not contain endotoxin) cause shock and multiple organ failure. We have discovered recently that two cell wall fragments of the pathogenic gram-positive bacterium Staphylococcus aureus, lipoteichoic acid (LTA) and peptidoglycan (PepG), synergize to cause the induction of nitric oxide (NO) formation, shock, and organ injury in the rat. We report here that a specific fragment of PepG, N-acetylglucosamine-β-[1→ 4]-N-acetylmuramyl-l-alanine–d-isoglutamine, is the moiety within the PepG polymer responsible for the synergism with LTA (or the cytokine interferon γ) to induce NO formation in the murine macrophage cell line J774.2. However, this moiety is also present in the PepG of the nonpathogenic bacterium Bacillus subtilis. We have discovered subsequently that S. aureus LTA synergizes with PepG from either bacterium to cause enhanced NO formation, shock, and organ injury in the rat, whereas the LTA from B. subtilis does not synergize with PepG of either bacterium. Thus, we propose that the structure of LTA determines the ability of a particular bacterium to cause shock and multiple organ failure (pathogenicity), while PepG acts to amplify any response induced by LTA.
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影响因子：
3.1
作者：
HEUMANN, D;BARRAS, C;TOMASZ, A
通讯作者：
TOMASZ, A
影响因子：
3.1
作者：
BHAKDI, S;MUHLY, M;HUGO, F
通讯作者：
HUGO, F
影响因子：
3.2
作者：
FOSTER, SJ
通讯作者：
FOSTER, SJ
DOI：
10.1099/00221287-139-11-2659
发表时间：
1993-11-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HIMANEN, JP;PYHALA, L;SARVAS, M
通讯作者：
SARVAS, M
影响因子：
5.5
作者：
CARD, GL;JASUJA, RR;GUSTAFSON, GL
通讯作者：
GUSTAFSON, GL